Design and synthesis of novel nucleotides for enzymatic gene synthesis

• Main Author: Ee, Pin Koon
• Other Authors: Chiba Shunsuke
• Format: Thesis-Doctor of Philosophy
• Language: English
• Published: Nanyang Technological University 2021
• Subjects: Science::Biological sciences::Genetics; Science::Chemistry
• Online Access: https://hdl.handle.net/10356/152672
• Institution: Nanyang Technological University

Traditionally, DNA was synthesized using a four-step chemical method based on phosphoramidite chemistry, allowing the synthesis of DNA strands up to 250-300 base pairs. However, this is insufficient for future application, such as DNA storage and gene synthesis. Alternatives to the phosphoramidite...