Identification of small molecules that interfere with c-di-GMP signaling and induce dispersal of Pseudomonas aeruginosa biofilms

Microbial biofilms are involved in a number of infections that cannot be cured, as microbes in biofilms resist host immune defenses and antibiotic therapies. With no strict biofilm-antibiotic in the current pipelines, there is an unmet need for drug candidates that enable the current antibiotics to...

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Main Authors: Andersen, Jens Bo, Hultqvist, Louise Dahl, Jansen, Charlotte Uldahl, Jakobsen, Tim Holm, Nilsson, Martin, Rybtke, Morten, Uhd, Jesper, Fritz, Blaine Gabriel, Seifert, Roland, Berthelsen, Jens, Nielsen, Thomas Eiland, Qvortrup, Katrine, Givskov, Michael, Tolker-Nielsen, Tim
Other Authors: Singapore Centre for Environmental Life Sciences and Engineering
Format: Article
Language:English
Published: 2021
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Online Access:https://hdl.handle.net/10356/153814
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spelling sg-ntu-dr.10356-1538142022-01-01T20:12:06Z Identification of small molecules that interfere with c-di-GMP signaling and induce dispersal of Pseudomonas aeruginosa biofilms Andersen, Jens Bo Hultqvist, Louise Dahl Jansen, Charlotte Uldahl Jakobsen, Tim Holm Nilsson, Martin Rybtke, Morten Uhd, Jesper Fritz, Blaine Gabriel Seifert, Roland Berthelsen, Jens Nielsen, Thomas Eiland Qvortrup, Katrine Givskov, Michael Tolker-Nielsen, Tim Singapore Centre for Environmental Life Sciences and Engineering Engineering::Environmental engineering Science::Biological sciences Antimicrobials Biofilms Microbial biofilms are involved in a number of infections that cannot be cured, as microbes in biofilms resist host immune defenses and antibiotic therapies. With no strict biofilm-antibiotic in the current pipelines, there is an unmet need for drug candidates that enable the current antibiotics to eradicate bacteria in biofilms. We used high-throughput screening to identify chemical compounds that reduce the intracellular c-di-GMP content in Pseudomonas aeruginosa. This led to the identification of a small molecule that efficiently depletes P. aeruginosa for c-di-GMP, inhibits biofilm formation, and disperses established biofilm. A combination of our lead compound with standard of care antibiotics showed improved eradication of an implant-associated infection established in mice. Genetic analyses provided evidence that the anti-biofilm compound stimulates the activity of the c-di-GMP phosphodiesterase BifA in P. aeruginosa. Our work constitutes a proof of concept for c-di-GMP phosphodiesterase-activating drugs administered in combination with antibiotics as a viable treatment strategy for otherwise recalcitrant infections. Published version This work was supported by grants to M.G. and T.T.N. from the Danish Council for Independent Research, the Lundbeck Foundation, the Novo Nordisk Foundation, and the Danish Ministry of Higher Education and Science (the DK-Openscreen program). Work by R.S. was supported by the Priority Programme “Nucleotide Second Messenger Signaling in Bacteria” (SPP 1879) of the Deutsche Forschungsgemeinschaft. We acknowledge NIH grant #P30DK089507 for the use of P. aeruginosa mutant strains from the University of Washington Transposon Mutant Collection. 2021-12-29T07:13:20Z 2021-12-29T07:13:20Z 2021 Journal Article Andersen, J. B., Hultqvist, L. D., Jansen, C. U., Jakobsen, T. H., Nilsson, M., Rybtke, M., Uhd, J., Fritz, B. G., Seifert, R., Berthelsen, J., Nielsen, T. E., Qvortrup, K., Givskov, M. & Tolker-Nielsen, T. (2021). Identification of small molecules that interfere with c-di-GMP signaling and induce dispersal of Pseudomonas aeruginosa biofilms. Npj Biofilms and Microbiomes, 7(1), 59-. https://dx.doi.org/10.1038/s41522-021-00225-4 2055-5008 https://hdl.handle.net/10356/153814 10.1038/s41522-021-00225-4 34244523 2-s2.0-85109786734 1 7 59 en npj Biofilms and Microbiomes © 2021 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering::Environmental engineering
Science::Biological sciences
Antimicrobials
Biofilms
spellingShingle Engineering::Environmental engineering
Science::Biological sciences
Antimicrobials
Biofilms
Andersen, Jens Bo
Hultqvist, Louise Dahl
Jansen, Charlotte Uldahl
Jakobsen, Tim Holm
Nilsson, Martin
Rybtke, Morten
Uhd, Jesper
Fritz, Blaine Gabriel
Seifert, Roland
Berthelsen, Jens
Nielsen, Thomas Eiland
Qvortrup, Katrine
Givskov, Michael
Tolker-Nielsen, Tim
Identification of small molecules that interfere with c-di-GMP signaling and induce dispersal of Pseudomonas aeruginosa biofilms
description Microbial biofilms are involved in a number of infections that cannot be cured, as microbes in biofilms resist host immune defenses and antibiotic therapies. With no strict biofilm-antibiotic in the current pipelines, there is an unmet need for drug candidates that enable the current antibiotics to eradicate bacteria in biofilms. We used high-throughput screening to identify chemical compounds that reduce the intracellular c-di-GMP content in Pseudomonas aeruginosa. This led to the identification of a small molecule that efficiently depletes P. aeruginosa for c-di-GMP, inhibits biofilm formation, and disperses established biofilm. A combination of our lead compound with standard of care antibiotics showed improved eradication of an implant-associated infection established in mice. Genetic analyses provided evidence that the anti-biofilm compound stimulates the activity of the c-di-GMP phosphodiesterase BifA in P. aeruginosa. Our work constitutes a proof of concept for c-di-GMP phosphodiesterase-activating drugs administered in combination with antibiotics as a viable treatment strategy for otherwise recalcitrant infections.
author2 Singapore Centre for Environmental Life Sciences and Engineering
author_facet Singapore Centre for Environmental Life Sciences and Engineering
Andersen, Jens Bo
Hultqvist, Louise Dahl
Jansen, Charlotte Uldahl
Jakobsen, Tim Holm
Nilsson, Martin
Rybtke, Morten
Uhd, Jesper
Fritz, Blaine Gabriel
Seifert, Roland
Berthelsen, Jens
Nielsen, Thomas Eiland
Qvortrup, Katrine
Givskov, Michael
Tolker-Nielsen, Tim
format Article
author Andersen, Jens Bo
Hultqvist, Louise Dahl
Jansen, Charlotte Uldahl
Jakobsen, Tim Holm
Nilsson, Martin
Rybtke, Morten
Uhd, Jesper
Fritz, Blaine Gabriel
Seifert, Roland
Berthelsen, Jens
Nielsen, Thomas Eiland
Qvortrup, Katrine
Givskov, Michael
Tolker-Nielsen, Tim
author_sort Andersen, Jens Bo
title Identification of small molecules that interfere with c-di-GMP signaling and induce dispersal of Pseudomonas aeruginosa biofilms
title_short Identification of small molecules that interfere with c-di-GMP signaling and induce dispersal of Pseudomonas aeruginosa biofilms
title_full Identification of small molecules that interfere with c-di-GMP signaling and induce dispersal of Pseudomonas aeruginosa biofilms
title_fullStr Identification of small molecules that interfere with c-di-GMP signaling and induce dispersal of Pseudomonas aeruginosa biofilms
title_full_unstemmed Identification of small molecules that interfere with c-di-GMP signaling and induce dispersal of Pseudomonas aeruginosa biofilms
title_sort identification of small molecules that interfere with c-di-gmp signaling and induce dispersal of pseudomonas aeruginosa biofilms
publishDate 2021
url https://hdl.handle.net/10356/153814
_version_ 1722355343826092032