Investigating non-nucleoside inhibitors targeting the Zika virus non-structural protein 5 (NS5) RNA-dependent RNA polymerase (RdRp) domain
Zika virus and members of the Flaviviridae family are re-emerging pathogens that pose a significant threat to public health. The lack of a vaccine and specific treatment of has resulted in a need for the design of therapeutic treatments to target specific parts of virus replication cycles. H...
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| Format: | Final Year Project |
| Language: | English |
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Nanyang Technological University
2022
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| Online Access: | https://hdl.handle.net/10356/157309 |
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| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | Zika virus and members of the Flaviviridae family are re-emerging pathogens that pose a
significant threat to public health. The lack of a vaccine and specific treatment of has resulted
in a need for the design of therapeutic treatments to target specific parts of virus replication
cycles.
Here, the RNA dependent RNA Polymerase (RdRP) of Zika Virus was purified based on
previously established protocols. Fluorescence-based alkaline phosphatase coupled
polymerase assay (FAPA), was used to screen a library of 86 compounds generated to target
the Zika Virus NS5 RdRP. Hit compounds identified were tested for dose-response
inhibition. Finally, crystallization efforts were carried out in hopes of obtaining crystal
structures of inhibitor compounds in complex with the Zika NS5 RdRp protein. |
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