Crystal structures of an HIV-1 integrase aptamer: formation of a water-mediated A•G•G•G•G pentad in an interlocked G-quadruplex

• Main Authors: Ngo, Khac Huy, Liew, Chong Wai, Lattmann, Simon, Winnerdy, Fernaldo Richtia, Phan, Anh Tuân
• Other Authors: School of Physical and Mathematical Sciences
• Format: Article
• Language: English
• Published: 2022
• Subjects: Science::Biological sciences; Science::Chemistry; G-Quadruplexes; Interlocked
• Online Access: https://hdl.handle.net/10356/158333
• Institution: Nanyang Technological University

93del is a 16-nucleotide G-quadruplex-forming aptamer which can inhibit the activity of the HIV-1 integrase enzyme at nanomolar concentration. Previous structural analyses of 93del using NMR spectroscopy have shown that the aptamer forms an interlocked G-quadruplex structure in K+ solution. Due to i...