Vypal2: a versatile peptide ligase for precision tailoring of proteins

• Main Authors: Zhang, Dingpeng, Wang, Zhen, Hu, Side, Lescar, Julien, Tam, James P., Liu, Chuan-Fa
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2022
• Subjects: Science::Biological sciences; Biocatalysts; Protein Labeling
• Online Access: https://hdl.handle.net/10356/160205
• Institution: Nanyang Technological University

The last two decades have seen an increasing demand for new protein-modification methods from the biotech industry and biomedical research communities. Owing to their mild aqueous reaction conditions, enzymatic methods based on the use of peptide ligases are particularly desirable. In this regard, t...