Decreased memory B cell frequencies in COVID-19 delta variant vaccine breakthrough infection

The SARS-CoV-2 Delta (B.1.617.2) variant is capable of infecting vaccinated persons. An open question remains as to whether deficiencies in specific vaccine-elicited immune responses result in susceptibility to vaccine breakthrough infection. We investigated 55 vaccine breakthrough infection cases (...

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Main Authors: Tay, Matthew Zirui, Rouers, Angeline, Fong, Siew-Wai, Goh, Yun Shan, Chan, Yi-Hao, Chang, Zi Wei, Xu, Weili, Tan, Chee Wah, Chia, Wan Ni, Torres-Ruesta, Anthony, Siti Naqiah Amrun, Huang, Yuling, Hor, Pei Xiang, Loh, Chiew Yee, Yeo, Nicholas Kim-Wah, Wang, Bei, Ngoh, Eve Zi Xian, Siti Nazihah Mohd Salleh, Chavatte, Jean-Marc, Lim, Alicia Jieling, Maurer-Stroh, Sebastian, Wang, Lin-Fa, Lin, Raymond Valentine Tzer Pin, Wang, Cheng-I, Tan, Seow-Yen, Young, Barnaby Edward, Leo, Yee Sin, Lye, David C., Renia, Laurent, Ng, Lisa F. P.
Other Authors: Lee Kong Chian School of Medicine (LKCMedicine)
Format: Article
Language:English
Published: 2022
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Online Access:https://hdl.handle.net/10356/163077
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1630772023-03-05T16:51:57Z Decreased memory B cell frequencies in COVID-19 delta variant vaccine breakthrough infection Tay, Matthew Zirui Rouers, Angeline Fong, Siew-Wai Goh, Yun Shan Chan, Yi-Hao Chang, Zi Wei Xu, Weili Tan, Chee Wah Chia, Wan Ni Torres-Ruesta, Anthony Siti Naqiah Amrun Huang, Yuling Hor, Pei Xiang Loh, Chiew Yee Yeo, Nicholas Kim-Wah Wang, Bei Ngoh, Eve Zi Xian Siti Nazihah Mohd Salleh Chavatte, Jean-Marc Lim, Alicia Jieling Maurer-Stroh, Sebastian Wang, Lin-Fa Lin, Raymond Valentine Tzer Pin Wang, Cheng-I Tan, Seow-Yen Young, Barnaby Edward Leo, Yee Sin Lye, David C. Renia, Laurent Ng, Lisa F. P. Lee Kong Chian School of Medicine (LKCMedicine) A*STAR Infectious Diseases Labs Singapore Immunology Network, A*STAR National Centre for Infectious Diseases, Singapore Tan Tock Seng Hospital Yong Loo Lin School of Medicine, NUS Science::Medicine Correlate of Risk COVID-19 The SARS-CoV-2 Delta (B.1.617.2) variant is capable of infecting vaccinated persons. An open question remains as to whether deficiencies in specific vaccine-elicited immune responses result in susceptibility to vaccine breakthrough infection. We investigated 55 vaccine breakthrough infection cases (mostly Delta) in Singapore, comparing them against 86 vaccinated close contacts who did not contract infection. Vaccine breakthrough cases showed lower memory B cell frequencies against SARS-CoV-2 receptor-binding domain (RBD). Compared to plasma antibodies, antibodies secreted by memory B cells retained a higher fraction of neutralizing properties against the Delta variant. Inflammatory cytokines including IL-1β and TNF were lower in vaccine breakthrough infections than primary infection of similar disease severity, underscoring the usefulness of vaccination in preventing inflammation. This report highlights the importance of memory B cells against vaccine breakthrough and suggests that lower memory B cell levels may be a correlate of risk for Delta vaccine breakthrough infection. Agency for Science, Technology and Research (A*STAR) National Medical Research Council (NMRC) National Research Foundation (NRF) Published version This work was supported by the Biomedical Research Council (BMRC), A*CRUSE (Vaccine monitoring project), the A*ccele-rate GAP-funded project (ACCL/19-GAP064-R20H-H) from Agency for Science, Technology and Research (A*STAR), Singapore National Medical Research Council COVID-19Research Fund (COVID19RF-001; COVID19RF-007;COVID19RF-008; COVID19RF-060; OF-LCG19May-0034) and A*STAR COVID-19 Research funding (H/20/04/g1/006). The SIgN Multiplex Analysis of Proteins (MAP) platform was supported by a grant from the National Research Foundation, Immunomonitoring Service Platform (ISP) (NRF2017_SISFP09). 2022-11-21T01:26:26Z 2022-11-21T01:26:26Z 2022 Journal Article Tay, M. Z., Rouers, A., Fong, S., Goh, Y. S., Chan, Y., Chang, Z. W., Xu, W., Tan, C. W., Chia, W. N., Torres-Ruesta, A., Siti Naqiah Amrun, Huang, Y., Hor, P. X., Loh, C. Y., Yeo, N. K., Wang, B., Ngoh, E. Z. X., Siti Nazihah Mohd Salleh, Chavatte, J., ...Ng, L. F. P. (2022). Decreased memory B cell frequencies in COVID-19 delta variant vaccine breakthrough infection. EMBO Molecular Medicine, 14(3), e15227-. https://dx.doi.org/10.15252/emmm.202115227 1757-4676 https://hdl.handle.net/10356/163077 10.15252/emmm.202115227 34994081 2-s2.0-85123235419 3 14 e15227 en ACCL/19-GAP064-R20H-H COVID19RF-001 COVID19RF-007 COVID19RF-008 COVID19RF-060 OF-LCG19May-0034 H/20/04/g1/006 NRF2017_SISFP09 EMBO Molecular Medicine © 2022 The Authors. Published under the terms of the CC BY 4.0 license. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Medicine
Correlate of Risk
COVID-19
spellingShingle Science::Medicine
Correlate of Risk
COVID-19
Tay, Matthew Zirui
Rouers, Angeline
Fong, Siew-Wai
Goh, Yun Shan
Chan, Yi-Hao
Chang, Zi Wei
Xu, Weili
Tan, Chee Wah
Chia, Wan Ni
Torres-Ruesta, Anthony
Siti Naqiah Amrun
Huang, Yuling
Hor, Pei Xiang
Loh, Chiew Yee
Yeo, Nicholas Kim-Wah
Wang, Bei
Ngoh, Eve Zi Xian
Siti Nazihah Mohd Salleh
Chavatte, Jean-Marc
Lim, Alicia Jieling
Maurer-Stroh, Sebastian
Wang, Lin-Fa
Lin, Raymond Valentine Tzer Pin
Wang, Cheng-I
Tan, Seow-Yen
Young, Barnaby Edward
Leo, Yee Sin
Lye, David C.
Renia, Laurent
Ng, Lisa F. P.
Decreased memory B cell frequencies in COVID-19 delta variant vaccine breakthrough infection
description The SARS-CoV-2 Delta (B.1.617.2) variant is capable of infecting vaccinated persons. An open question remains as to whether deficiencies in specific vaccine-elicited immune responses result in susceptibility to vaccine breakthrough infection. We investigated 55 vaccine breakthrough infection cases (mostly Delta) in Singapore, comparing them against 86 vaccinated close contacts who did not contract infection. Vaccine breakthrough cases showed lower memory B cell frequencies against SARS-CoV-2 receptor-binding domain (RBD). Compared to plasma antibodies, antibodies secreted by memory B cells retained a higher fraction of neutralizing properties against the Delta variant. Inflammatory cytokines including IL-1β and TNF were lower in vaccine breakthrough infections than primary infection of similar disease severity, underscoring the usefulness of vaccination in preventing inflammation. This report highlights the importance of memory B cells against vaccine breakthrough and suggests that lower memory B cell levels may be a correlate of risk for Delta vaccine breakthrough infection.
author2 Lee Kong Chian School of Medicine (LKCMedicine)
author_facet Lee Kong Chian School of Medicine (LKCMedicine)
Tay, Matthew Zirui
Rouers, Angeline
Fong, Siew-Wai
Goh, Yun Shan
Chan, Yi-Hao
Chang, Zi Wei
Xu, Weili
Tan, Chee Wah
Chia, Wan Ni
Torres-Ruesta, Anthony
Siti Naqiah Amrun
Huang, Yuling
Hor, Pei Xiang
Loh, Chiew Yee
Yeo, Nicholas Kim-Wah
Wang, Bei
Ngoh, Eve Zi Xian
Siti Nazihah Mohd Salleh
Chavatte, Jean-Marc
Lim, Alicia Jieling
Maurer-Stroh, Sebastian
Wang, Lin-Fa
Lin, Raymond Valentine Tzer Pin
Wang, Cheng-I
Tan, Seow-Yen
Young, Barnaby Edward
Leo, Yee Sin
Lye, David C.
Renia, Laurent
Ng, Lisa F. P.
format Article
author Tay, Matthew Zirui
Rouers, Angeline
Fong, Siew-Wai
Goh, Yun Shan
Chan, Yi-Hao
Chang, Zi Wei
Xu, Weili
Tan, Chee Wah
Chia, Wan Ni
Torres-Ruesta, Anthony
Siti Naqiah Amrun
Huang, Yuling
Hor, Pei Xiang
Loh, Chiew Yee
Yeo, Nicholas Kim-Wah
Wang, Bei
Ngoh, Eve Zi Xian
Siti Nazihah Mohd Salleh
Chavatte, Jean-Marc
Lim, Alicia Jieling
Maurer-Stroh, Sebastian
Wang, Lin-Fa
Lin, Raymond Valentine Tzer Pin
Wang, Cheng-I
Tan, Seow-Yen
Young, Barnaby Edward
Leo, Yee Sin
Lye, David C.
Renia, Laurent
Ng, Lisa F. P.
author_sort Tay, Matthew Zirui
title Decreased memory B cell frequencies in COVID-19 delta variant vaccine breakthrough infection
title_short Decreased memory B cell frequencies in COVID-19 delta variant vaccine breakthrough infection
title_full Decreased memory B cell frequencies in COVID-19 delta variant vaccine breakthrough infection
title_fullStr Decreased memory B cell frequencies in COVID-19 delta variant vaccine breakthrough infection
title_full_unstemmed Decreased memory B cell frequencies in COVID-19 delta variant vaccine breakthrough infection
title_sort decreased memory b cell frequencies in covid-19 delta variant vaccine breakthrough infection
publishDate 2022
url https://hdl.handle.net/10356/163077
_version_ 1759852928957415424