Arl15 upregulates the TGFβ family signaling by promoting the assembly of the Smad-complex
The hallmark event of the canonical transforming growth factor β (TGFβ) family signaling is the assembly of the Smad-complex, consisting of the common Smad, Smad4, and phos-phorylated receptor-regulated Smads. How the Smad-complex is assembled and regulated is still unclear. Here, we report that act...
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sg-ntu-dr.10356-1636692023-06-13T03:24:37Z Arl15 upregulates the TGFβ family signaling by promoting the assembly of the Smad-complex Shi, Meng Tie, Hieng Chiong Divyanshu, Mahajan Sun, Xiuping Zhou, Yan Boh, Boon Kim Vardy, Leah A. Lu, Lei School of Biological Sciences Science::Biological sciences Transactivator Protein Signal Transduction The hallmark event of the canonical transforming growth factor β (TGFβ) family signaling is the assembly of the Smad-complex, consisting of the common Smad, Smad4, and phos-phorylated receptor-regulated Smads. How the Smad-complex is assembled and regulated is still unclear. Here, we report that active Arl15, an Arf-like small G protein, specifically binds to the MH2 domain of Smad4 and colocalizes with Smad4 at the endolysosome. The binding relieves the auto-inhibition of Smad4, which is imposed by the intramolecular interaction between its MH1 and MH2 domains. Activated Smad4 subsequently interacts with phosphorylated receptor-regulated Smads, forming the Smad-complex. Our observations suggest that Smad4 functions as an effector and a GTPase activating protein (GAP) of Arl15. Assembly of the Smad-complex enhances the GAP activity of Smad4 toward Arl15, therefore dissociating Arl15 before the nuclear translocation of the Smad-complex. Our data further demonstrate that Arl15 positively regulates the TGFβ family signaling. Ministry of Education (MOE) Published version This work was supported by the following grants: MOE AcRF Tier1 RG35/17, Tier2 MOE2015-T2-2-073, and MOE2018-T2-2-026. 2022-12-19T00:35:48Z 2022-12-19T00:35:48Z 2022 Journal Article Shi, M., Tie, H. C., Divyanshu, M., Sun, X., Zhou, Y., Boh, B. K., Vardy, L. A. & Lu, L. (2022). Arl15 upregulates the TGFβ family signaling by promoting the assembly of the Smad-complex. ELife, 11, e76146-. https://dx.doi.org/10.7554/ELIFE.76146 2050-084X https://hdl.handle.net/10356/163669 10.7554/ELIFE.76146 11 2-s2.0-85135596538 11 e76146 en AcRF Tier1 RG35/17 Tier2 MOE2015-T2-2-073 MOE2018-T2-2-026. eLife 10.21979/N9/WRFNC2 © Shi et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. application/pdf |
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Science::Biological sciences Transactivator Protein Signal Transduction Shi, Meng Tie, Hieng Chiong Divyanshu, Mahajan Sun, Xiuping Zhou, Yan Boh, Boon Kim Vardy, Leah A. Lu, Lei Arl15 upregulates the TGFβ family signaling by promoting the assembly of the Smad-complex |
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The hallmark event of the canonical transforming growth factor β (TGFβ) family signaling is the assembly of the Smad-complex, consisting of the common Smad, Smad4, and phos-phorylated receptor-regulated Smads. How the Smad-complex is assembled and regulated is still unclear. Here, we report that active Arl15, an Arf-like small G protein, specifically binds to the MH2 domain of Smad4 and colocalizes with Smad4 at the endolysosome. The binding relieves the auto-inhibition of Smad4, which is imposed by the intramolecular interaction between its MH1 and MH2 domains. Activated Smad4 subsequently interacts with phosphorylated receptor-regulated Smads, forming the Smad-complex. Our observations suggest that Smad4 functions as an effector and a GTPase activating protein (GAP) of Arl15. Assembly of the Smad-complex enhances the GAP activity of Smad4 toward Arl15, therefore dissociating Arl15 before the nuclear translocation of the Smad-complex. Our data further demonstrate that Arl15 positively regulates the TGFβ family signaling. |
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School of Biological Sciences |
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School of Biological Sciences Shi, Meng Tie, Hieng Chiong Divyanshu, Mahajan Sun, Xiuping Zhou, Yan Boh, Boon Kim Vardy, Leah A. Lu, Lei |
format |
Article |
author |
Shi, Meng Tie, Hieng Chiong Divyanshu, Mahajan Sun, Xiuping Zhou, Yan Boh, Boon Kim Vardy, Leah A. Lu, Lei |
author_sort |
Shi, Meng |
title |
Arl15 upregulates the TGFβ family signaling by promoting the assembly of the Smad-complex |
title_short |
Arl15 upregulates the TGFβ family signaling by promoting the assembly of the Smad-complex |
title_full |
Arl15 upregulates the TGFβ family signaling by promoting the assembly of the Smad-complex |
title_fullStr |
Arl15 upregulates the TGFβ family signaling by promoting the assembly of the Smad-complex |
title_full_unstemmed |
Arl15 upregulates the TGFβ family signaling by promoting the assembly of the Smad-complex |
title_sort |
arl15 upregulates the tgfβ family signaling by promoting the assembly of the smad-complex |
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2022 |
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https://hdl.handle.net/10356/163669 |
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1772826414761902080 |