Mitofusins Mfn1 and Mfn2 are required to preserve glucose- but not incretin-stimulated β-cell connectivity and insulin secretion
Mitochondrial glucose metabolism is essential for stimulated insulin release from pancreatic β-cells. Whether mitofusin gene expression, and hence, mitochondrial network integrity, is important for glucose or incretin signaling has not previously been explored. Here, we generated mice with β-cell-se...
Saved in:
Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , |
---|---|
Other Authors: | |
Format: | Article |
Language: | English |
Published: |
2022
|
Subjects: | |
Online Access: | https://hdl.handle.net/10356/163808 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Nanyang Technological University |
Language: | English |
id |
sg-ntu-dr.10356-163808 |
---|---|
record_format |
dspace |
institution |
Nanyang Technological University |
building |
NTU Library |
continent |
Asia |
country |
Singapore Singapore |
content_provider |
NTU Library |
collection |
DR-NTU |
language |
English |
topic |
Science::Medicine Adenosine Triphosphate Diabetes Mellitus |
spellingShingle |
Science::Medicine Adenosine Triphosphate Diabetes Mellitus Georgiadou, Eleni Muralidharan, Charanya Martinez, Michelle Chabosseau, Pauline Akalestou, Elina Tomas, Alejandra Yong, Fiona Su Wern Stylianides, Theodoros Wretlind, Asger Legido-Quigley, Cristina Jones, Ben Lopez-Noriega, Livia Xu, Yanwen Gu, Guoqiang Alsabeeh, Nour Cruciani-Guglielmacci, Céline Magnan, Christophe Ibberson, Mark Leclerc, Isabelle Ali, Yusuf Soleimanpour, Scott A. Linnemann, Amelia K. Rodriguez, Tristan A. Rutter, Guy A. Mitofusins Mfn1 and Mfn2 are required to preserve glucose- but not incretin-stimulated β-cell connectivity and insulin secretion |
description |
Mitochondrial glucose metabolism is essential for stimulated insulin release from pancreatic β-cells. Whether mitofusin gene expression, and hence, mitochondrial network integrity, is important for glucose or incretin signaling has not previously been explored. Here, we generated mice with β-cell-selective, adult-restricted deletion knock-out (dKO) of the mitofusin genes Mfn1 and Mfn2 (βMfn1/2 dKO). βMfn1/2-dKO mice displayed elevated fed and fasted glycemia and a more than fivefold decrease in plasma insulin. Mitochondrial length, glucose-induced polarization, ATP synthesis, and cytosolic and mitochondrial Ca2+ increases were all reduced in dKO islets. In contrast, oral glucose tolerance was more modestly affected in βMfn1/2-dKO mice, and glucagon-like peptide 1 or glucose-dependent insulinotropic peptide receptor agonists largely corrected defective glucose-stimulated insulin secretion through enhanced EPAC-dependent signaling. Correspondingly, cAMP increases in the cytosol, as measured with an Epac-camps-based sensor, were exaggerated in dKO mice. Mitochondrial fusion and fission cycles are thus essential in the β-cell to maintain normal glucose, but not incretin, sensing. These findings broaden our understanding of the roles of mitofusins in β-cells, the potential contributions of altered mitochondrial dynamics to diabetes development, and the impact of incretins on this process. |
author2 |
Lee Kong Chian School of Medicine (LKCMedicine) |
author_facet |
Lee Kong Chian School of Medicine (LKCMedicine) Georgiadou, Eleni Muralidharan, Charanya Martinez, Michelle Chabosseau, Pauline Akalestou, Elina Tomas, Alejandra Yong, Fiona Su Wern Stylianides, Theodoros Wretlind, Asger Legido-Quigley, Cristina Jones, Ben Lopez-Noriega, Livia Xu, Yanwen Gu, Guoqiang Alsabeeh, Nour Cruciani-Guglielmacci, Céline Magnan, Christophe Ibberson, Mark Leclerc, Isabelle Ali, Yusuf Soleimanpour, Scott A. Linnemann, Amelia K. Rodriguez, Tristan A. Rutter, Guy A. |
format |
Article |
author |
Georgiadou, Eleni Muralidharan, Charanya Martinez, Michelle Chabosseau, Pauline Akalestou, Elina Tomas, Alejandra Yong, Fiona Su Wern Stylianides, Theodoros Wretlind, Asger Legido-Quigley, Cristina Jones, Ben Lopez-Noriega, Livia Xu, Yanwen Gu, Guoqiang Alsabeeh, Nour Cruciani-Guglielmacci, Céline Magnan, Christophe Ibberson, Mark Leclerc, Isabelle Ali, Yusuf Soleimanpour, Scott A. Linnemann, Amelia K. Rodriguez, Tristan A. Rutter, Guy A. |
author_sort |
Georgiadou, Eleni |
title |
Mitofusins Mfn1 and Mfn2 are required to preserve glucose- but not incretin-stimulated β-cell connectivity and insulin secretion |
title_short |
Mitofusins Mfn1 and Mfn2 are required to preserve glucose- but not incretin-stimulated β-cell connectivity and insulin secretion |
title_full |
Mitofusins Mfn1 and Mfn2 are required to preserve glucose- but not incretin-stimulated β-cell connectivity and insulin secretion |
title_fullStr |
Mitofusins Mfn1 and Mfn2 are required to preserve glucose- but not incretin-stimulated β-cell connectivity and insulin secretion |
title_full_unstemmed |
Mitofusins Mfn1 and Mfn2 are required to preserve glucose- but not incretin-stimulated β-cell connectivity and insulin secretion |
title_sort |
mitofusins mfn1 and mfn2 are required to preserve glucose- but not incretin-stimulated β-cell connectivity and insulin secretion |
publishDate |
2022 |
url |
https://hdl.handle.net/10356/163808 |
_version_ |
1753801181951426560 |
spelling |
sg-ntu-dr.10356-1638082022-12-19T02:20:45Z Mitofusins Mfn1 and Mfn2 are required to preserve glucose- but not incretin-stimulated β-cell connectivity and insulin secretion Georgiadou, Eleni Muralidharan, Charanya Martinez, Michelle Chabosseau, Pauline Akalestou, Elina Tomas, Alejandra Yong, Fiona Su Wern Stylianides, Theodoros Wretlind, Asger Legido-Quigley, Cristina Jones, Ben Lopez-Noriega, Livia Xu, Yanwen Gu, Guoqiang Alsabeeh, Nour Cruciani-Guglielmacci, Céline Magnan, Christophe Ibberson, Mark Leclerc, Isabelle Ali, Yusuf Soleimanpour, Scott A. Linnemann, Amelia K. Rodriguez, Tristan A. Rutter, Guy A. Lee Kong Chian School of Medicine (LKCMedicine) Science::Medicine Adenosine Triphosphate Diabetes Mellitus Mitochondrial glucose metabolism is essential for stimulated insulin release from pancreatic β-cells. Whether mitofusin gene expression, and hence, mitochondrial network integrity, is important for glucose or incretin signaling has not previously been explored. Here, we generated mice with β-cell-selective, adult-restricted deletion knock-out (dKO) of the mitofusin genes Mfn1 and Mfn2 (βMfn1/2 dKO). βMfn1/2-dKO mice displayed elevated fed and fasted glycemia and a more than fivefold decrease in plasma insulin. Mitochondrial length, glucose-induced polarization, ATP synthesis, and cytosolic and mitochondrial Ca2+ increases were all reduced in dKO islets. In contrast, oral glucose tolerance was more modestly affected in βMfn1/2-dKO mice, and glucagon-like peptide 1 or glucose-dependent insulinotropic peptide receptor agonists largely corrected defective glucose-stimulated insulin secretion through enhanced EPAC-dependent signaling. Correspondingly, cAMP increases in the cytosol, as measured with an Epac-camps-based sensor, were exaggerated in dKO mice. Mitochondrial fusion and fission cycles are thus essential in the β-cell to maintain normal glucose, but not incretin, sensing. These findings broaden our understanding of the roles of mitofusins in β-cells, the potential contributions of altered mitochondrial dynamics to diabetes development, and the impact of incretins on this process. G.A.R. was supported by a Wellcome Trust Senior Investigator Award (098424AIA) and Wellcome Trust Investigator Award (212625/Z/18/Z), Medical Research Council Programme grants (MR/R022259/1, MR/J0003042/ 1, MR/L020149/1), an Experimental Challenge Grant (DIVA, MR/L02036X/1), a Medical Research Council grant (MR/N00275X/1), and Diabetes UK grants (BDA/11/0004210, BDA/15/0005275, BDA16/0005485). I.L. was supported by a Diabetes UKD project grant (16/0005485). This project has received funding from the European Commission Innovative Medicines Initiative 2 Joint Undertaking, under grant agreement no. 115881 (RHAPSODY). This Joint Undertaking receives support from the European Union’s Horizon 2020 Research and Innovation Programme. This work is supported by the Swiss State Secretariat for Education, Research and Innovation (SERI), under contract no. 16.0097. A.T. was supported by Medical Research Council project grant MR/R010676/ 1. Intravital imaging was performed using resources and/or funding provided by National Institutes of Health grants R03 DK115990 (to A.K.L.), Human Islet Research Network UC4 DK104162 (to A.K.L., RRID:SCR_014393). BJ acknowledges support from the Academy of Medical Sciences, Society for Endocrinology, The British Society for Neuroendocrinology, the European Federation for the Study of Diabetes, an Engineering and Physical Sciences Research Council capital award, and the Medical Research Council (MR/R010676/1). S.A.S. was supported by the JDRF (CDA-2016-189, SRA-2018-539, COE-2019-861), the National Institutes of Health (R01 DK108921, U01 DK127747), and the U.S. Department of Veterans Affairs (I01 BX004444). 2022-12-19T02:20:45Z 2022-12-19T02:20:45Z 2022 Journal Article Georgiadou, E., Muralidharan, C., Martinez, M., Chabosseau, P., Akalestou, E., Tomas, A., Yong, F. S. W., Stylianides, T., Wretlind, A., Legido-Quigley, C., Jones, B., Lopez-Noriega, L., Xu, Y., Gu, G., Alsabeeh, N., Cruciani-Guglielmacci, C., Magnan, C., Ibberson, M., Leclerc, I., ...Rutter, G. A. (2022). Mitofusins Mfn1 and Mfn2 are required to preserve glucose- but not incretin-stimulated β-cell connectivity and insulin secretion. Diabetes, 71(7), 1472-1489. https://dx.doi.org/10.2337/db21-0800 0012-1797 https://hdl.handle.net/10356/163808 10.2337/db21-0800 35472764 2-s2.0-85133103048 7 71 1472 1489 en Diabetes © 2022 by the American Diabetes Association. All rights reserved. |