Automatic DNA replication tract measurement to assess replication and repair dynamics at the single-molecule level

• Main Authors: Li, Longjie, Kolinjivadi, Arun Mouli, Ong, Kok Haur, Young, David M., Marini, Gabriel Pik Liang, Chan, Sock Hoai, Chong, Siao Ting, Chew, Ee Ling, Lu, Haoda, Gole, Laurent, Yu, Weimiao, Ngeow, Joanne
• Other Authors: Lee Kong Chian School of Medicine (LKCMedicine)
• Format: Article
• Language: English
• Published: 2023
• Subjects: Science::Medicine; Sofware; Replication
• Online Access: https://hdl.handle.net/10356/164980
• Institution: Nanyang Technological University

DNA fibre assay has a potential application in genomic medicine, cancer and stem cell research at the single-molecule level. A major challenge for the clinical and research implementation of DNA fibre assays is the slow speed in which manual analysis takes place as it limits the clinical actionability. While automatic detection of DNA fibres speeds up this process considerably, current publicly available software have limited features in terms of their user interface for manual correction of results, which in turn limit their accuracy and ability to account for atypical structures that may be important in diagnosis or investigative studies. We recognize that core improvements can be made to the GUI to allow for direct interaction with automatic results to preserve accuracy as well as enhance the versatility of automatic DNA fibre detection for use in variety of situations.