Reconstituted TAD-size chromatin fibers feature heterogeneous nucleosome clusters

• Main Authors: Korolev, Nikolay, Zinchenko, Anatoly, Soman, Aghil, Chen, Qinming, Wong, Sook Yi, Berezhnoy, Nikolay V., Basak, Rajib, van der Maarel, Johan R. C., van Noort, John, Nordenskiöld, Lars
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2023
• Subjects: Science::Biological sciences; Histone; Chromatin
• Online Access: https://hdl.handle.net/10356/165451
• Institution: Nanyang Technological University

Large topologically associated domains (TADs) contain irregularly spaced nucleosome clutches, and interactions between such clutches are thought to aid the compaction of these domains. Here, we reconstituted TAD-sized chromatin fibers containing hundreds of nucleosomes on native source human and lam...