SRRM2 organizes splicing condensates to regulate alternative splicing

• Main Authors: Xu, Shaohai, Lai, Soak-Kuan, Sim, Donald Yuhui, Ang, Warren Shou Leong, Li, Hoi Yeung, Roca, Xavier
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2023
• Subjects: Science::Biological sciences; Acute Myeloid Leukemia; Alternative RNA Splicing
• Online Access: https://hdl.handle.net/10356/168783
• Institution: Nanyang Technological University

SRRM2 is a nuclear-speckle marker containing multiple disordered domains, whose dysfunction is associated with several human diseases. Using mainly EGFP-SRRM2 knock-in HEK293T cells, we show that SRRM2 forms biomolecular condensates satisfying most hallmarks of liquid-liquid phase separation, includ...