The respiratory syncytial virus SH protein is incorporated into infectious virus particles that form on virus-infected cells

• Main Authors: Huong, Tra Nguyen, Iyer, Laxmi Ravi, Lui, Jing, Wang, De Yun, Tan, Boon Huan, Sugrue, Richard J.
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2023
• Subjects: Science::Biological sciences; Respiratory Syncytial Virus; SH Protein
• Online Access: https://hdl.handle.net/10356/168919
• Institution: Nanyang Technological University

The association of the SH protein with respiratory syncytial virus (RSV) particles was examined in HEp2 cells and human ciliated nasal epithelial cells. Imaging of infected cells demonstrated the presence of the SH protein in virus filaments, and analysis of purified RSV particles revealed a SH protein species whose size was consistent with the glycosylated SH protein. Although the SH protein was detected in virus filaments it was not required for virus filament formation. Analysis of RSV-infected ciliated cells also revealed that the SH protein was trafficked into the cilia, and this correlated with reduced cilia density on these cells. Reduced cilia loss was not observed on ciliated cells infected with a RSV isolate that failed to express the SH protein. These data provide direct evidence that the SH protein is trafficked into virus particles, and suggests that the SH protein may also promote cilia dysfunction on nasal epithelial cells.