DOK3 promotes atopic dermatitis by enabling the phosphatase PP4C to inhibit the T cell signaling mediator CARD11
The scaffolding protein CARD11 is a critical mediator of antigen receptor signaling in lymphocytes. Hypomorphic (partial loss-of-function) mutations in CARD11 are associated with the development of severe atopic dermatitis, in which T cell receptor signaling is reduced and helper T cell differentiat...
Saved in:
Main Authors: | , , , , , , |
---|---|
Other Authors: | |
Format: | Article |
Language: | English |
Published: |
2024
|
Subjects: | |
Online Access: | https://hdl.handle.net/10356/173519 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Nanyang Technological University |
Language: | English |
id |
sg-ntu-dr.10356-173519 |
---|---|
record_format |
dspace |
spelling |
sg-ntu-dr.10356-1735192024-02-09T02:27:47Z DOK3 promotes atopic dermatitis by enabling the phosphatase PP4C to inhibit the T cell signaling mediator CARD11 Loh Jia Tong Teo, Joey Kay Hui Kannan, Srinivasaraghavan Verma, Chandra Shekhar Andiappan, Anand Kumar Lim, Hong-Hwa Lam, Kong-Peng School of Biological Sciences Mathematical Sciences Adaptor Proteins Phosphoric Monoester Hydrolases The scaffolding protein CARD11 is a critical mediator of antigen receptor signaling in lymphocytes. Hypomorphic (partial loss-of-function) mutations in CARD11 are associated with the development of severe atopic dermatitis, in which T cell receptor signaling is reduced and helper T cell differentiation is skewed to an allergy-associated type 2 phenotype. Here, we found that the docking protein DOK3 plays a key role in the pathogenesis of atopic dermatitis by suppressing CARD11 activity. DOK3 interacted with CARD11 and decreased its phosphorylation in T cells by recruiting the catalytic subunit of protein phosphatase 4, thereby dampening downstream signaling. Knocking out Dok3 enhanced the production of the cytokine IFN-γ by T cells, which conferred protection against experimental atopic dermatitis-like skin inflammation in mice. The expression of DOK3 was increased in T cells isolated from patients with atopic dermatitis and inversely correlated with IFNG expression. A subset of hypomorphic CARD11 variants found in patients with atopic dermatitis bound more strongly than wild-type CARD11 to DOK3. Our findings suggest that the strength of the interaction of DOK3 with CARD11 may predispose individuals to developing atopic dermatitis. Ministry of Health (MOH) National Medical Research Council (NMRC) his work is supportedby the Singapore Ministry of Health’sNationalMedicalResearch Councilunderits Open-Fund-IndividualResearch Grant (NMRC/OFIRG19may-0083)to K.-P.L., H.-H.L.,and J.T.L.; Open Fund-Young IndividualResearch Grant(NMRC/OFYIRG21nov-0035)to J.T.L.; A*STAR core grant to K.-P.L.; and A*STAR CareerDevelopmentAward (202D800012)to A.K.A. 2024-02-09T02:26:40Z 2024-02-09T02:26:40Z 2023 Journal Article Loh Jia Tong, Teo, J. K. H., Kannan, S., Verma, C. S., Andiappan, A. K., Lim, H. & Lam, K. (2023). DOK3 promotes atopic dermatitis by enabling the phosphatase PP4C to inhibit the T cell signaling mediator CARD11. Science Signaling, 16(809), eadg5171-. https://dx.doi.org/10.1126/scisignal.adg5171 1945-0877 https://hdl.handle.net/10356/173519 10.1126/scisignal.adg5171 37906628 2-s2.0-85175581587 809 16 eadg5171 en MRC/OFIRG19may-0083 NMRC/OFYIRG21nov-0035 202D800012 Science Signaling © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. |
institution |
Nanyang Technological University |
building |
NTU Library |
continent |
Asia |
country |
Singapore Singapore |
content_provider |
NTU Library |
collection |
DR-NTU |
language |
English |
topic |
Mathematical Sciences Adaptor Proteins Phosphoric Monoester Hydrolases |
spellingShingle |
Mathematical Sciences Adaptor Proteins Phosphoric Monoester Hydrolases Loh Jia Tong Teo, Joey Kay Hui Kannan, Srinivasaraghavan Verma, Chandra Shekhar Andiappan, Anand Kumar Lim, Hong-Hwa Lam, Kong-Peng DOK3 promotes atopic dermatitis by enabling the phosphatase PP4C to inhibit the T cell signaling mediator CARD11 |
description |
The scaffolding protein CARD11 is a critical mediator of antigen receptor signaling in lymphocytes. Hypomorphic (partial loss-of-function) mutations in CARD11 are associated with the development of severe atopic dermatitis, in which T cell receptor signaling is reduced and helper T cell differentiation is skewed to an allergy-associated type 2 phenotype. Here, we found that the docking protein DOK3 plays a key role in the pathogenesis of atopic dermatitis by suppressing CARD11 activity. DOK3 interacted with CARD11 and decreased its phosphorylation in T cells by recruiting the catalytic subunit of protein phosphatase 4, thereby dampening downstream signaling. Knocking out Dok3 enhanced the production of the cytokine IFN-γ by T cells, which conferred protection against experimental atopic dermatitis-like skin inflammation in mice. The expression of DOK3 was increased in T cells isolated from patients with atopic dermatitis and inversely correlated with IFNG expression. A subset of hypomorphic CARD11 variants found in patients with atopic dermatitis bound more strongly than wild-type CARD11 to DOK3. Our findings suggest that the strength of the interaction of DOK3 with CARD11 may predispose individuals to developing atopic dermatitis. |
author2 |
School of Biological Sciences |
author_facet |
School of Biological Sciences Loh Jia Tong Teo, Joey Kay Hui Kannan, Srinivasaraghavan Verma, Chandra Shekhar Andiappan, Anand Kumar Lim, Hong-Hwa Lam, Kong-Peng |
format |
Article |
author |
Loh Jia Tong Teo, Joey Kay Hui Kannan, Srinivasaraghavan Verma, Chandra Shekhar Andiappan, Anand Kumar Lim, Hong-Hwa Lam, Kong-Peng |
author_sort |
Loh Jia Tong |
title |
DOK3 promotes atopic dermatitis by enabling the phosphatase PP4C to inhibit the T cell signaling mediator CARD11 |
title_short |
DOK3 promotes atopic dermatitis by enabling the phosphatase PP4C to inhibit the T cell signaling mediator CARD11 |
title_full |
DOK3 promotes atopic dermatitis by enabling the phosphatase PP4C to inhibit the T cell signaling mediator CARD11 |
title_fullStr |
DOK3 promotes atopic dermatitis by enabling the phosphatase PP4C to inhibit the T cell signaling mediator CARD11 |
title_full_unstemmed |
DOK3 promotes atopic dermatitis by enabling the phosphatase PP4C to inhibit the T cell signaling mediator CARD11 |
title_sort |
dok3 promotes atopic dermatitis by enabling the phosphatase pp4c to inhibit the t cell signaling mediator card11 |
publishDate |
2024 |
url |
https://hdl.handle.net/10356/173519 |
_version_ |
1794549472160645120 |