Recombinant perlecan domain V covalently immobilized on silk biomaterials via plasma immersion ion implantation supports the formation of functional endothelium
Strategies to promote rapid formation of functional endothelium are required to maintain blood fluidity and regulate smooth muscle cell proliferation in synthetic vascular conduits. In this work, we explored the biofunctionalization of silk biomaterials with recombinantly expressed domain V of human...
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sg-ntu-dr.10356-1745752024-04-05T15:31:52Z Recombinant perlecan domain V covalently immobilized on silk biomaterials via plasma immersion ion implantation supports the formation of functional endothelium Lau, Kieran Fu, Lu Zhang, Anyu Akhavan, Behnam Whitelock, John Bilek, Marcela M. Lord, Megan S. Rnjak-Kovacina, Jelena School of Chemistry, Chemical Engineering and Biotechnology Engineering Biofunctionalization Endothelium Strategies to promote rapid formation of functional endothelium are required to maintain blood fluidity and regulate smooth muscle cell proliferation in synthetic vascular conduits. In this work, we explored the biofunctionalization of silk biomaterials with recombinantly expressed domain V of human perlecan (rDV) to promote endothelial cell interactions and the formation of functional endothelium. Perlecan is essential in vascular development and homeostasis and rDV has been shown to uniquely support endothelial cell, while inhibiting smooth muscle cell and platelet interactions, both key contributors of vascular graft failure. rDV was covalently immobilized on silk using plasma immersion ion implantation (PIII), a simple one-step surface treatment process which enables strong immobilization in the absence of chemical cross-linkers. rDV immobilization on surface-modified silk was assessed for amount, orientation, and bio-functionality in terms of endothelial cell interactions and functional endothelial layer formation. rDV immobilized on PIII-treated silk (rDV-PIII-silk) supported rapid endothelial cell adhesion, spreading, and proliferation to form functional endothelium, as evidenced by the expression of vinculin and VE-cadherin markers. Taken together, the results provide evidence for the potential of rDV-PIII-silk as a biomimetic vascular graft material. Published version The authors would like to acknowledge funding support from the Australian Research Council (LP180100540, FT2101000668; Jelena Rnjak‐Kovacina and LP190101003; Megan S. Lord). Kieran Lau was supported by the Australian Government Research Training Program Scholarship. Confocal microscopy was partially performed using instruments situated in, and maintained by, the Katharina Gaus Light Microscopy Facility. Katharina Gaus Light Microscopy Facility is part of the Mark Wainwright Analytical Center at UNSW Sydney which is in part‐funded by the Research Infrastructure program at UNSW. Open access publishing facilitated by University of New South Wales, as part of the Wiley ‐ University of New South Wales agreement via the Council of Australian University Librarians. 2024-04-03T01:18:45Z 2024-04-03T01:18:45Z 2023 Journal Article Lau, K., Fu, L., Zhang, A., Akhavan, B., Whitelock, J., Bilek, M. M., Lord, M. S. & Rnjak-Kovacina, J. (2023). Recombinant perlecan domain V covalently immobilized on silk biomaterials via plasma immersion ion implantation supports the formation of functional endothelium. Journal of Biomedical Materials Research Part A, 111(6), 825-839. https://dx.doi.org/10.1002/jbm.a.37525 1549-3296 https://hdl.handle.net/10356/174575 10.1002/jbm.a.37525 36897070 2-s2.0-85150611228 6 111 825 839 en Journal of Biomedical Materials Research Part A © 2023 The Authors. Journal of Biomedical Materials Research Part A published by Wiley Periodicals LLC. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. application/pdf |
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Engineering Biofunctionalization Endothelium Lau, Kieran Fu, Lu Zhang, Anyu Akhavan, Behnam Whitelock, John Bilek, Marcela M. Lord, Megan S. Rnjak-Kovacina, Jelena Recombinant perlecan domain V covalently immobilized on silk biomaterials via plasma immersion ion implantation supports the formation of functional endothelium |
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Strategies to promote rapid formation of functional endothelium are required to maintain blood fluidity and regulate smooth muscle cell proliferation in synthetic vascular conduits. In this work, we explored the biofunctionalization of silk biomaterials with recombinantly expressed domain V of human perlecan (rDV) to promote endothelial cell interactions and the formation of functional endothelium. Perlecan is essential in vascular development and homeostasis and rDV has been shown to uniquely support endothelial cell, while inhibiting smooth muscle cell and platelet interactions, both key contributors of vascular graft failure. rDV was covalently immobilized on silk using plasma immersion ion implantation (PIII), a simple one-step surface treatment process which enables strong immobilization in the absence of chemical cross-linkers. rDV immobilization on surface-modified silk was assessed for amount, orientation, and bio-functionality in terms of endothelial cell interactions and functional endothelial layer formation. rDV immobilized on PIII-treated silk (rDV-PIII-silk) supported rapid endothelial cell adhesion, spreading, and proliferation to form functional endothelium, as evidenced by the expression of vinculin and VE-cadherin markers. Taken together, the results provide evidence for the potential of rDV-PIII-silk as a biomimetic vascular graft material. |
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School of Chemistry, Chemical Engineering and Biotechnology |
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School of Chemistry, Chemical Engineering and Biotechnology Lau, Kieran Fu, Lu Zhang, Anyu Akhavan, Behnam Whitelock, John Bilek, Marcela M. Lord, Megan S. Rnjak-Kovacina, Jelena |
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Article |
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Lau, Kieran Fu, Lu Zhang, Anyu Akhavan, Behnam Whitelock, John Bilek, Marcela M. Lord, Megan S. Rnjak-Kovacina, Jelena |
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Lau, Kieran |
title |
Recombinant perlecan domain V covalently immobilized on silk biomaterials via plasma immersion ion implantation supports the formation of functional endothelium |
title_short |
Recombinant perlecan domain V covalently immobilized on silk biomaterials via plasma immersion ion implantation supports the formation of functional endothelium |
title_full |
Recombinant perlecan domain V covalently immobilized on silk biomaterials via plasma immersion ion implantation supports the formation of functional endothelium |
title_fullStr |
Recombinant perlecan domain V covalently immobilized on silk biomaterials via plasma immersion ion implantation supports the formation of functional endothelium |
title_full_unstemmed |
Recombinant perlecan domain V covalently immobilized on silk biomaterials via plasma immersion ion implantation supports the formation of functional endothelium |
title_sort |
recombinant perlecan domain v covalently immobilized on silk biomaterials via plasma immersion ion implantation supports the formation of functional endothelium |
publishDate |
2024 |
url |
https://hdl.handle.net/10356/174575 |
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1806059779107323904 |