Mechanistic basis for potassium efflux-driven activation of the human NLRP1 inflammasome

• Main Authors: Rozario, Pritisha, Pinilla, Miriam, Gorse, Leana, Vind, Anna Constance, Robinson, Kim S., Toh, Gee Ann, Muhammad Jasrie Firdaus, Martínez, José Francisco, Kerk, Swat Kim, Lin, Zhewang, Chambers, John Campbell, Bekker-Jensen, Simon, Meunier, Etienne, Zhong, Franklin
• Other Authors: Lee Kong Chian School of Medicine (LKCMedicine)
• Format: Article
• Language: English
• Published: 2024
• Subjects: Medicine, Health and Life Sciences; Autophosphorylation; Bright field microscopy
• Online Access: https://hdl.handle.net/10356/176261
• Institution: Nanyang Technological University

Nigericin, an ionophore derived from Streptomyces hygroscopicus, is arguably the most commonly used tool compound to study the NLRP3 inflammasome. Recent findings, however, showed that nigericin also activates the NLRP1 inflammasome in human keratinocytes. In this study, we resolve the mechanistic b...