Functional characterization of human respiratory syncytial virus (hRSV) small hydrophobic protein (SH) through small interfering RNA (siRNA) that targets SH.

Small hydrophobic (SH) protein is a transmembrane protein from Human Respiratory Syncytial Virus (hRSV). Though its gene sequence is highly conserved in clinical isolates, the function remains debatable in viral pathogenesis and replication. In this study, small interfering RNA (siRNA) targeting SH...

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Main Author: Ngan, Grace Jie Yin.
Other Authors: Richard J. Sugrue
Format: Final Year Project
Language:English
Published: 2009
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Online Access:http://hdl.handle.net/10356/19055
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-190552023-02-28T18:07:57Z Functional characterization of human respiratory syncytial virus (hRSV) small hydrophobic protein (SH) through small interfering RNA (siRNA) that targets SH. Ngan, Grace Jie Yin. Richard J. Sugrue School of Biological Sciences DRNTU::Science::Biological sciences::Microbiology::Virology Small hydrophobic (SH) protein is a transmembrane protein from Human Respiratory Syncytial Virus (hRSV). Though its gene sequence is highly conserved in clinical isolates, the function remains debatable in viral pathogenesis and replication. In this study, small interfering RNA (siRNA) targeting SH was employed to examine the effect of SH gene silencing and protein knockdown to other viral proteins – Fusion (F), Attachment (G) protein and Phosphopeotein (P) during viral pathogenesis in Hep-2 cell line. SH knockdown did not noticeably affect virus replication or result in a significant change in the distribution of viral proteins. However, subtle changes in the level of surface biotinylated F and G were observed in SH siRNA- treated sample. While immunoprecipitation using SH antibody showed a slight decrease of cross-linked surface G protein upon SH knockdown, SH knockdown however did not abolish the G proteins surface assembly, suggesting that SH may be involved indirectly in the process of a subpopulations SH-G complex formation on the surface of RSV-infected cells. SH seems to be dispensable for virus growth in tissue culture and further study is required to elucidate the function of SH during host-virus interaction. Bachelor of Science in Biological Sciences 2009-09-16T01:28:34Z 2009-09-16T01:28:34Z 2009 2009 Final Year Project (FYP) http://hdl.handle.net/10356/19055 en Nanyang Technological University 34 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Microbiology::Virology
spellingShingle DRNTU::Science::Biological sciences::Microbiology::Virology
Ngan, Grace Jie Yin.
Functional characterization of human respiratory syncytial virus (hRSV) small hydrophobic protein (SH) through small interfering RNA (siRNA) that targets SH.
description Small hydrophobic (SH) protein is a transmembrane protein from Human Respiratory Syncytial Virus (hRSV). Though its gene sequence is highly conserved in clinical isolates, the function remains debatable in viral pathogenesis and replication. In this study, small interfering RNA (siRNA) targeting SH was employed to examine the effect of SH gene silencing and protein knockdown to other viral proteins – Fusion (F), Attachment (G) protein and Phosphopeotein (P) during viral pathogenesis in Hep-2 cell line. SH knockdown did not noticeably affect virus replication or result in a significant change in the distribution of viral proteins. However, subtle changes in the level of surface biotinylated F and G were observed in SH siRNA- treated sample. While immunoprecipitation using SH antibody showed a slight decrease of cross-linked surface G protein upon SH knockdown, SH knockdown however did not abolish the G proteins surface assembly, suggesting that SH may be involved indirectly in the process of a subpopulations SH-G complex formation on the surface of RSV-infected cells. SH seems to be dispensable for virus growth in tissue culture and further study is required to elucidate the function of SH during host-virus interaction.
author2 Richard J. Sugrue
author_facet Richard J. Sugrue
Ngan, Grace Jie Yin.
format Final Year Project
author Ngan, Grace Jie Yin.
author_sort Ngan, Grace Jie Yin.
title Functional characterization of human respiratory syncytial virus (hRSV) small hydrophobic protein (SH) through small interfering RNA (siRNA) that targets SH.
title_short Functional characterization of human respiratory syncytial virus (hRSV) small hydrophobic protein (SH) through small interfering RNA (siRNA) that targets SH.
title_full Functional characterization of human respiratory syncytial virus (hRSV) small hydrophobic protein (SH) through small interfering RNA (siRNA) that targets SH.
title_fullStr Functional characterization of human respiratory syncytial virus (hRSV) small hydrophobic protein (SH) through small interfering RNA (siRNA) that targets SH.
title_full_unstemmed Functional characterization of human respiratory syncytial virus (hRSV) small hydrophobic protein (SH) through small interfering RNA (siRNA) that targets SH.
title_sort functional characterization of human respiratory syncytial virus (hrsv) small hydrophobic protein (sh) through small interfering rna (sirna) that targets sh.
publishDate 2009
url http://hdl.handle.net/10356/19055
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