Examination of effect of thymine loop length on polymorphism of G-quadruplex.
Structural polymorphism is one of the important issue with regard to G-quadruplexes because the structural diversity may significantly affect their biological functions in vivo and also their physical properties in nano-material. A series of oligonucleotides with...
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Final Year Project |
| Language: | English |
| Published: |
2010
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/10356/38630 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | Structural polymorphism is one of the important issue with regard to G-quadruplexes because the structural diversity may significantly affect their biological functions in vivo and also their physical properties in nano-material. A series of oligonucleotides with four repeat guanines sequences, d(G4Tn)3G4 (where n = 1-6) were designed for this study to investigate the effect of the length of loop on the polymorphism of G-quadruplex and the effect of the structure of the resulting G-quadruplex on its melting point. Via CD spectroscopy and comparison with the known CD spectrums of previously studied G-quadruplexes, and with the help of PAGE analysis, the structures of the G-quadruplexes could be predicted. A trend was also observed for these oligonucleotides while varying their T-loop length and this will be analyzed in this study. |
|---|