Potential role of novel PDZK1 adaptor protein in adipocyte development and lipid metabolism.
Research on adipogenesis has garnered great interest as adipose tissue was identified to possess endocrinal activities, other than just a depot for fats storage. We have identified a novel PSD-95/Dlg/ZO-1 domain containing 1 (PDZK1) adaptor protein in adipocyte that was downregulated when the...
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Format: | Final Year Project |
Language: | English |
Published: |
2011
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Online Access: | http://hdl.handle.net/10356/44744 |
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Institution: | Nanyang Technological University |
Language: | English |
Summary: | Research on adipogenesis has garnered great interest as adipose tissue was identified to
possess endocrinal activities, other than just a depot for fats storage. We have identified a
novel PSD-95/Dlg/ZO-1 domain containing 1 (PDZK1) adaptor protein in adipocyte that was
downregulated when the lipodystrophic gene, Seipin, was mutated. PDZK1 role in adipocyte
was unknown and we sought to elucidate its expression and regulatory function during
adipogenesis. PDZK1 expression in 3T3-L1 cells was observed to be highly upregulated in
late differentiation and was tightly dependent on adipocyte differentiation, similar to the
expression pattern of key adipogenic regulators. This suggests a potential novel role for
PDZK1 in regulating late stage adipocyte differentiation. PDZK1 reported receptor target
gene in liver, Scavenger Receptor Class B Type I (SR-BI), was also highly upregulated
during late differentiation in adipocyte. This strengthened our hypothesis that they may
together regulate terminal adipocyte differentiation. SR-BI and key pro-adipogenic regulators
such as CCAAT/Enhancer Binding Protein-α, Peroxisome Proliferator-Activated Receptors-γ
and Sterol Response Element-Binding-Protein 1c were not downregulated, when PDZK1 was
knock-down by short-hairpin RNA. Whether PDZK1 act upstream or downstream of these
pro-adipogenic factors requires further test as our control cells did not differentiate well to
provide an accurate comparison. |
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