Optimization of SMART-based PCR for amplification from picogram amounts of RNA.

• المؤلف الرئيسي: Lin, Zhaoting.
• مؤلفون آخرون: Zbynek Bozdech
• التنسيق: Final Year Project
• اللغة: English
• منشور في: 2011
• الموضوعات: DRNTU::Science::Biological sciences::Genetics
• الوصول للمادة أونلاين: http://hdl.handle.net/10356/45664
• المؤسسة: Nanyang Technological University

One of the technical issues faced in malaria research involving transcriptional profiling and analysis using microarrays is the difficulty faced in obtaining adequate biological material from patients for field isolate studies. To overcome the problem, a SMART-based PCR technique is optimized to acc...