Optimization of SMART-based PCR for amplification from picogram amounts of RNA.

• Main Author: Lin, Zhaoting.
• Other Authors: Zbynek Bozdech
• Format: Final Year Project
• Language: English
• Published: 2011
• Subjects: DRNTU::Science::Biological sciences::Genetics
• Online Access: http://hdl.handle.net/10356/45664
• Institution: Nanyang Technological University

One of the technical issues faced in malaria research involving transcriptional profiling and analysis using microarrays is the difficulty faced in obtaining adequate biological material from patients for field isolate studies. To overcome the problem, a SMART-based PCR technique is optimized to acc...