Using single-molecule techniques to study DNA mechanics and DNA-protein interactions
This work focuses on two single-molecule techniques: Magnetic Tweezers and Tethered Particle Motion (TPM). Magnetic Tweezers were used to study the DNA overstretching transition from the B-form to the S-form at 65 pN. The operations of the magnetic tweezers were studied and the resolution of its mea...
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| Format: | Final Year Project |
| Language: | English |
| Published: |
2012
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| Online Access: | http://hdl.handle.net/10356/49068 |
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| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | This work focuses on two single-molecule techniques: Magnetic Tweezers and Tethered Particle Motion (TPM). Magnetic Tweezers were used to study the DNA overstretching transition from the B-form to the S-form at 65 pN. The operations of the magnetic tweezers were studied and the resolution of its measurements discussed. G-Quadruplex DNA constructs were designed and magnetic tweezers experiments were done on one of them. The preliminary data shows no detectable signal from G-Quadruplex. TPM was developed and fundamental experiments were done to demonstrate its capabilities. Its future improvements and applications were proposed and discussed. |
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