Using single-molecule techniques to study DNA mechanics and DNA-protein interactions

Using single-molecule techniques to study DNA mechanics and DNA-protein interactions

This work focuses on two single-molecule techniques: Magnetic Tweezers and Tethered Particle Motion (TPM). Magnetic Tweezers were used to study the DNA overstretching transition from the B-form to the S-form at 65 pN. The operations of the magnetic tweezers were studied and the resolution of its mea...

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Main Author: Fang, Keyi
Other Authors: Phan Anh Tuan
Format: Final Year Project
Language:English
Published: 2012
Subjects:
DRNTU::Science::Biological sciences::Biophysics
Online Access:http://hdl.handle.net/10356/49068
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-490682023-02-28T23:19:27Z Using single-molecule techniques to study DNA mechanics and DNA-protein interactions Fang, Keyi Phan Anh Tuan School of Physical and Mathematical Sciences DRNTU::Science::Biological sciences::Biophysics This work focuses on two single-molecule techniques: Magnetic Tweezers and Tethered Particle Motion (TPM). Magnetic Tweezers were used to study the DNA overstretching transition from the B-form to the S-form at 65 pN. The operations of the magnetic tweezers were studied and the resolution of its measurements discussed. G-Quadruplex DNA constructs were designed and magnetic tweezers experiments were done on one of them. The preliminary data shows no detectable signal from G-Quadruplex. TPM was developed and fundamental experiments were done to demonstrate its capabilities. Its future improvements and applications were proposed and discussed. Bachelor of Science in Physics 2012-05-14T07:53:44Z 2012-05-14T07:53:44Z 2012 2012 Final Year Project (FYP) http://hdl.handle.net/10356/49068 en 96 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Biophysics
spellingShingle DRNTU::Science::Biological sciences::Biophysics
Fang, Keyi
Using single-molecule techniques to study DNA mechanics and DNA-protein interactions
description This work focuses on two single-molecule techniques: Magnetic Tweezers and Tethered Particle Motion (TPM). Magnetic Tweezers were used to study the DNA overstretching transition from the B-form to the S-form at 65 pN. The operations of the magnetic tweezers were studied and the resolution of its measurements discussed. G-Quadruplex DNA constructs were designed and magnetic tweezers experiments were done on one of them. The preliminary data shows no detectable signal from G-Quadruplex. TPM was developed and fundamental experiments were done to demonstrate its capabilities. Its future improvements and applications were proposed and discussed.
author2 Phan Anh Tuan
author_facet Phan Anh Tuan
Fang, Keyi
format Final Year Project
author Fang, Keyi
author_sort Fang, Keyi
title Using single-molecule techniques to study DNA mechanics and DNA-protein interactions
title_short Using single-molecule techniques to study DNA mechanics and DNA-protein interactions
title_full Using single-molecule techniques to study DNA mechanics and DNA-protein interactions
title_fullStr Using single-molecule techniques to study DNA mechanics and DNA-protein interactions
title_full_unstemmed Using single-molecule techniques to study DNA mechanics and DNA-protein interactions
title_sort using single-molecule techniques to study dna mechanics and dna-protein interactions
publishDate 2012
url http://hdl.handle.net/10356/49068
_version_ 1759858159845900288

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