Functional amyloids in Pseudomonas aeruginosa may be involved in the sequestration of the quorum sensing molecules HHQ and PQS under high shear conditions.
Pseudomonas aeruginosa is an opportunistic pathogen that causes chronic infections in cystic fibrosis patients. In the airways of the lungs, fluids are flowing at inconsistent rate and this could dilute concentrations of signalling molecules and nutrients that are important to the pathogen. These si...
Saved in:
Main Author: | |
---|---|
Other Authors: | |
Format: | Final Year Project |
Language: | English |
Published: |
2013
|
Subjects: | |
Online Access: | http://hdl.handle.net/10356/53796 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Nanyang Technological University |
Language: | English |
id |
sg-ntu-dr.10356-53796 |
---|---|
record_format |
dspace |
spelling |
sg-ntu-dr.10356-537962023-02-28T18:01:28Z Functional amyloids in Pseudomonas aeruginosa may be involved in the sequestration of the quorum sensing molecules HHQ and PQS under high shear conditions. Sia, Wan Rong. School of Biological Sciences Thomas Seviour DRNTU::Science::Biological sciences::Microbiology Pseudomonas aeruginosa is an opportunistic pathogen that causes chronic infections in cystic fibrosis patients. In the airways of the lungs, fluids are flowing at inconsistent rate and this could dilute concentrations of signalling molecules and nutrients that are important to the pathogen. These signalling molecules regulate the formation of biofilm and production of virulence factors, hence playing a role in its pathogenicity. We hypothesize that P.aeruginosa express amyloid-like fimbrae, Fap, to bind to these molecules to prevent their loss into the environment. The binding affinities of Fap to Quorum Sensing (QS) molecules were investigated using Surface Plasmon Reasonance and a bioreporter assay. Results from the investigations revealed that Fap binds to QS molecules at high affinities and was able to retain them under high shear force. Therefore, we propose that Fap has a functional role in sequestering QS molecules under high shear conditions. Bachelor of Science in Biological Sciences 2013-06-07T06:38:58Z 2013-06-07T06:38:58Z 2013 2013 Final Year Project (FYP) http://hdl.handle.net/10356/53796 en Nanyang Technological University 34 p. application/pdf |
institution |
Nanyang Technological University |
building |
NTU Library |
continent |
Asia |
country |
Singapore Singapore |
content_provider |
NTU Library |
collection |
DR-NTU |
language |
English |
topic |
DRNTU::Science::Biological sciences::Microbiology |
spellingShingle |
DRNTU::Science::Biological sciences::Microbiology Sia, Wan Rong. Functional amyloids in Pseudomonas aeruginosa may be involved in the sequestration of the quorum sensing molecules HHQ and PQS under high shear conditions. |
description |
Pseudomonas aeruginosa is an opportunistic pathogen that causes chronic infections in cystic fibrosis patients. In the airways of the lungs, fluids are flowing at inconsistent rate and this could dilute concentrations of signalling molecules and nutrients that are important to the pathogen. These signalling molecules regulate the formation of biofilm and production of virulence factors, hence playing a role in its pathogenicity. We hypothesize that P.aeruginosa express amyloid-like fimbrae, Fap, to bind to these molecules to prevent their loss into the environment. The binding affinities of Fap to Quorum Sensing (QS) molecules were investigated using Surface Plasmon Reasonance and a bioreporter assay. Results from the investigations revealed that Fap binds to QS molecules at high affinities and was able to retain them under high shear force. Therefore, we propose that Fap has a functional role in sequestering QS molecules under high shear conditions. |
author2 |
School of Biological Sciences |
author_facet |
School of Biological Sciences Sia, Wan Rong. |
format |
Final Year Project |
author |
Sia, Wan Rong. |
author_sort |
Sia, Wan Rong. |
title |
Functional amyloids in Pseudomonas aeruginosa may be involved in the sequestration of the quorum sensing molecules HHQ and PQS under high shear conditions. |
title_short |
Functional amyloids in Pseudomonas aeruginosa may be involved in the sequestration of the quorum sensing molecules HHQ and PQS under high shear conditions. |
title_full |
Functional amyloids in Pseudomonas aeruginosa may be involved in the sequestration of the quorum sensing molecules HHQ and PQS under high shear conditions. |
title_fullStr |
Functional amyloids in Pseudomonas aeruginosa may be involved in the sequestration of the quorum sensing molecules HHQ and PQS under high shear conditions. |
title_full_unstemmed |
Functional amyloids in Pseudomonas aeruginosa may be involved in the sequestration of the quorum sensing molecules HHQ and PQS under high shear conditions. |
title_sort |
functional amyloids in pseudomonas aeruginosa may be involved in the sequestration of the quorum sensing molecules hhq and pqs under high shear conditions. |
publishDate |
2013 |
url |
http://hdl.handle.net/10356/53796 |
_version_ |
1759857773325058048 |