Functional amyloids in Pseudomonas aeruginosa may be involved in the sequestration of the quorum sensing molecules HHQ and PQS under high shear conditions.

Pseudomonas aeruginosa is an opportunistic pathogen that causes chronic infections in cystic fibrosis patients. In the airways of the lungs, fluids are flowing at inconsistent rate and this could dilute concentrations of signalling molecules and nutrients that are important to the pathogen. These si...

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Main Author: Sia, Wan Rong.
Other Authors: School of Biological Sciences
Format: Final Year Project
Language:English
Published: 2013
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Online Access:http://hdl.handle.net/10356/53796
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-537962023-02-28T18:01:28Z Functional amyloids in Pseudomonas aeruginosa may be involved in the sequestration of the quorum sensing molecules HHQ and PQS under high shear conditions. Sia, Wan Rong. School of Biological Sciences Thomas Seviour DRNTU::Science::Biological sciences::Microbiology Pseudomonas aeruginosa is an opportunistic pathogen that causes chronic infections in cystic fibrosis patients. In the airways of the lungs, fluids are flowing at inconsistent rate and this could dilute concentrations of signalling molecules and nutrients that are important to the pathogen. These signalling molecules regulate the formation of biofilm and production of virulence factors, hence playing a role in its pathogenicity. We hypothesize that P.aeruginosa express amyloid-like fimbrae, Fap, to bind to these molecules to prevent their loss into the environment. The binding affinities of Fap to Quorum Sensing (QS) molecules were investigated using Surface Plasmon Reasonance and a bioreporter assay. Results from the investigations revealed that Fap binds to QS molecules at high affinities and was able to retain them under high shear force. Therefore, we propose that Fap has a functional role in sequestering QS molecules under high shear conditions. Bachelor of Science in Biological Sciences 2013-06-07T06:38:58Z 2013-06-07T06:38:58Z 2013 2013 Final Year Project (FYP) http://hdl.handle.net/10356/53796 en Nanyang Technological University 34 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Microbiology
spellingShingle DRNTU::Science::Biological sciences::Microbiology
Sia, Wan Rong.
Functional amyloids in Pseudomonas aeruginosa may be involved in the sequestration of the quorum sensing molecules HHQ and PQS under high shear conditions.
description Pseudomonas aeruginosa is an opportunistic pathogen that causes chronic infections in cystic fibrosis patients. In the airways of the lungs, fluids are flowing at inconsistent rate and this could dilute concentrations of signalling molecules and nutrients that are important to the pathogen. These signalling molecules regulate the formation of biofilm and production of virulence factors, hence playing a role in its pathogenicity. We hypothesize that P.aeruginosa express amyloid-like fimbrae, Fap, to bind to these molecules to prevent their loss into the environment. The binding affinities of Fap to Quorum Sensing (QS) molecules were investigated using Surface Plasmon Reasonance and a bioreporter assay. Results from the investigations revealed that Fap binds to QS molecules at high affinities and was able to retain them under high shear force. Therefore, we propose that Fap has a functional role in sequestering QS molecules under high shear conditions.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Sia, Wan Rong.
format Final Year Project
author Sia, Wan Rong.
author_sort Sia, Wan Rong.
title Functional amyloids in Pseudomonas aeruginosa may be involved in the sequestration of the quorum sensing molecules HHQ and PQS under high shear conditions.
title_short Functional amyloids in Pseudomonas aeruginosa may be involved in the sequestration of the quorum sensing molecules HHQ and PQS under high shear conditions.
title_full Functional amyloids in Pseudomonas aeruginosa may be involved in the sequestration of the quorum sensing molecules HHQ and PQS under high shear conditions.
title_fullStr Functional amyloids in Pseudomonas aeruginosa may be involved in the sequestration of the quorum sensing molecules HHQ and PQS under high shear conditions.
title_full_unstemmed Functional amyloids in Pseudomonas aeruginosa may be involved in the sequestration of the quorum sensing molecules HHQ and PQS under high shear conditions.
title_sort functional amyloids in pseudomonas aeruginosa may be involved in the sequestration of the quorum sensing molecules hhq and pqs under high shear conditions.
publishDate 2013
url http://hdl.handle.net/10356/53796
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