Quantification of AMY1 gene copy number and its association with glycemic response.

There has been no study to date which measures salivary amylase AMY1 gene copy number variation (CNV) in an Asian population, with varying prevalence of obesity and Type 2 Diabetes Mellitus (T2DM). The aim of this study is to investigate how the differences in CNV of AMY1 between individuals can inf...

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Main Author: Lee, Jin Shun.
Other Authors: Surajit Bhattacharyya
Format: Final Year Project
Language:English
Published: 2013
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Online Access:http://hdl.handle.net/10356/54805
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-548052023-02-28T18:02:54Z Quantification of AMY1 gene copy number and its association with glycemic response. Lee, Jin Shun. Surajit Bhattacharyya School of Biological Sciences A*STAR Singapore Institute for Clinical Sciences Lee Yung Seng Tan Verena Ooi, Delicia Shu Qin DRNTU::Science There has been no study to date which measures salivary amylase AMY1 gene copy number variation (CNV) in an Asian population, with varying prevalence of obesity and Type 2 Diabetes Mellitus (T2DM). The aim of this study is to investigate how the differences in CNV of AMY1 between individuals can influence postprandial glycemic response (GR), which is a well recognized and established risk factor that precipitates the development of T2DM. Healthy, non-obese, young men were recruited and asked to consume glucose solution (GS) (50g of anhydrous glucose dissolved in 250ml of water) as reference food and 50g of available carbohydrate from basmati (BR) or jasmine rice (JR) as test food, after fasted for 10 to 12 hour overnight. Buccal swabs and capillary bloods were taken for the measurement of AMY1 gene copy numbers and postprandial GR respectively. From the small number (n=9) of subjects that we analyzed, we found no significant correlation between subjects' AMY1 gene copy number and postprandial mean incremental Area under Curve (iAUC) values. Further analysis with a larger number of subjects is warranted for a statistically significant conclusion to be made. We recommend comparison between different ethnic groups to be made when sufficient subjects are recruited. Bachelor of Science in Biomedical Sciences 2013-08-22T06:26:29Z 2013-08-22T06:26:29Z 2013 2013 Final Year Project (FYP) http://hdl.handle.net/10356/54805 en Nanyang Technological University 35 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science
spellingShingle DRNTU::Science
Lee, Jin Shun.
Quantification of AMY1 gene copy number and its association with glycemic response.
description There has been no study to date which measures salivary amylase AMY1 gene copy number variation (CNV) in an Asian population, with varying prevalence of obesity and Type 2 Diabetes Mellitus (T2DM). The aim of this study is to investigate how the differences in CNV of AMY1 between individuals can influence postprandial glycemic response (GR), which is a well recognized and established risk factor that precipitates the development of T2DM. Healthy, non-obese, young men were recruited and asked to consume glucose solution (GS) (50g of anhydrous glucose dissolved in 250ml of water) as reference food and 50g of available carbohydrate from basmati (BR) or jasmine rice (JR) as test food, after fasted for 10 to 12 hour overnight. Buccal swabs and capillary bloods were taken for the measurement of AMY1 gene copy numbers and postprandial GR respectively. From the small number (n=9) of subjects that we analyzed, we found no significant correlation between subjects' AMY1 gene copy number and postprandial mean incremental Area under Curve (iAUC) values. Further analysis with a larger number of subjects is warranted for a statistically significant conclusion to be made. We recommend comparison between different ethnic groups to be made when sufficient subjects are recruited.
author2 Surajit Bhattacharyya
author_facet Surajit Bhattacharyya
Lee, Jin Shun.
format Final Year Project
author Lee, Jin Shun.
author_sort Lee, Jin Shun.
title Quantification of AMY1 gene copy number and its association with glycemic response.
title_short Quantification of AMY1 gene copy number and its association with glycemic response.
title_full Quantification of AMY1 gene copy number and its association with glycemic response.
title_fullStr Quantification of AMY1 gene copy number and its association with glycemic response.
title_full_unstemmed Quantification of AMY1 gene copy number and its association with glycemic response.
title_sort quantification of amy1 gene copy number and its association with glycemic response.
publishDate 2013
url http://hdl.handle.net/10356/54805
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