The role of growth factor receptor bound protein 2 (GRB2) in gain of function of mutant p53.

• Main Author: Lau, Sok Ting.
• Other Authors: Surajit Bhattacharyya
• Format: Final Year Project
• Language: English
• Published: 2013
• Subjects: DRNTU::Science::Biological sciences::Human anatomy and physiology
• Online Access: http://hdl.handle.net/10356/54806
• Institution: Nanyang Technological University

Mutation and inactivation of p53 is almost a universal feature in human cancer. That has made p53 one of the most studied proteins. Several mutant p53 have been shown to have dominant phenotype over wild-type p53. Studies have attributed the ability of mutant p53 to acquire oncogenic properties such as cell proliferation, metastasis as a “gain-of-function”. In this study, GRB2, a binding partner of mutant p53 was explored to uncover its role in mutant p53 “gain-of-function”. In this study, it was ascertained that the binding of GRB2 and mutant p53 occurs in the nucleus which is interesting as GRB2 usually localize in the cytoplasm. In addition, GRB2 was also discovered to bind to the DNA binding domain on mutant p53. Functional assays with regard, to cell proliferation and motility can be done in the future to determine the purpose of GRB2 and mutant p53 interaction.