Proteomics analysis of an UBE3A knockout mouse modelling Angelman syndrome
Angelman syndrome is a neurobehavioral disease associated with the loss of maternally expressed E3 ubiquitin protein ligase, Ube3a. Ube3a gene is biallelically expressed in all tissue except cerebellum, Purkinje cells and hippocampus, in which Ube3a is expressed exclusively from maternally inherited...
Saved in:
Main Author: | |
---|---|
Other Authors: | |
Format: | Theses and Dissertations |
Language: | English |
Published: |
2014
|
Subjects: | |
Online Access: | https://hdl.handle.net/10356/55407 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Nanyang Technological University |
Language: | English |
id |
sg-ntu-dr.10356-55407 |
---|---|
record_format |
dspace |
spelling |
sg-ntu-dr.10356-554072023-02-28T18:47:09Z Proteomics analysis of an UBE3A knockout mouse modelling Angelman syndrome Low, Hai Loon Chen Ken-Shiung School of Biological Sciences DRNTU::Science::Biological sciences Angelman syndrome is a neurobehavioral disease associated with the loss of maternally expressed E3 ubiquitin protein ligase, Ube3a. Ube3a gene is biallelically expressed in all tissue except cerebellum, Purkinje cells and hippocampus, in which Ube3a is expressed exclusively from maternally inherited chromosome. By using an Ube3a knockout mouse model, the effect of loss of Ube3a in brain has been studied using 2-D DIGE method. Due to the fact that Ube3a is involved in ubiquitin-related proteasomal degradation, its substrates and downstream targets are expected to be differentially expressed in knockout and wild-type models. A total of 94 proteins from cerebellum and 74 proteins from hippocampus were found differentially expressed in the Ube3a knockout mice using 2-D DIGE, 14 of them were statistically significant. Western blot and Real Time RT PCR were then employed to examine the protein level and mRNA level of those proteins, respectively. CaBP was found downregulated at mRNA as well as protein level; its function as calcium ion buffer may play an inductive role in the seizure observed in AS mouse model and patients. DOCTOR OF PHILOSOPHY (SBS) 2014-02-28T01:13:27Z 2014-02-28T01:13:27Z 2014 2014 Thesis Low, H. L. (2014). Proteomics analysis of an UBE3A knockout mouse modelling angelman syndrome. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/55407 10.32657/10356/55407 en 138 p. application/pdf |
institution |
Nanyang Technological University |
building |
NTU Library |
continent |
Asia |
country |
Singapore Singapore |
content_provider |
NTU Library |
collection |
DR-NTU |
language |
English |
topic |
DRNTU::Science::Biological sciences |
spellingShingle |
DRNTU::Science::Biological sciences Low, Hai Loon Proteomics analysis of an UBE3A knockout mouse modelling Angelman syndrome |
description |
Angelman syndrome is a neurobehavioral disease associated with the loss of maternally expressed E3 ubiquitin protein ligase, Ube3a. Ube3a gene is biallelically expressed in all tissue except cerebellum, Purkinje cells and hippocampus, in which Ube3a is expressed exclusively from maternally inherited chromosome. By using an Ube3a knockout mouse model, the effect of loss of Ube3a in brain has been studied using 2-D DIGE method. Due to the fact that Ube3a is involved in ubiquitin-related proteasomal degradation, its substrates and downstream targets are expected to be differentially expressed in knockout and wild-type models. A total of 94 proteins from cerebellum and 74 proteins from hippocampus were found differentially expressed in the Ube3a knockout mice using 2-D DIGE, 14 of them were statistically significant. Western blot and Real Time RT PCR were then employed to examine the protein level and mRNA level of those proteins, respectively. CaBP was found downregulated at mRNA as well as protein level; its function as calcium ion buffer may play an inductive role in the seizure observed in AS mouse model and patients. |
author2 |
Chen Ken-Shiung |
author_facet |
Chen Ken-Shiung Low, Hai Loon |
format |
Theses and Dissertations |
author |
Low, Hai Loon |
author_sort |
Low, Hai Loon |
title |
Proteomics analysis of an UBE3A knockout mouse modelling Angelman syndrome |
title_short |
Proteomics analysis of an UBE3A knockout mouse modelling Angelman syndrome |
title_full |
Proteomics analysis of an UBE3A knockout mouse modelling Angelman syndrome |
title_fullStr |
Proteomics analysis of an UBE3A knockout mouse modelling Angelman syndrome |
title_full_unstemmed |
Proteomics analysis of an UBE3A knockout mouse modelling Angelman syndrome |
title_sort |
proteomics analysis of an ube3a knockout mouse modelling angelman syndrome |
publishDate |
2014 |
url |
https://hdl.handle.net/10356/55407 |
_version_ |
1759857328902897664 |