Elucidating the role of SCD1 in B cell development and function

• Main Author: Loo, Larry Sai Weng
• Other Authors: Lam Kong Peng
• Format: Theses and Dissertations
• Language: English
• Published: 2015
• Subjects: DRNTU::Science::Biological sciences::Molecular biology
• Online Access: http://hdl.handle.net/10356/63295
• Institution: Nanyang Technological University

Stearoyl-coenzyme A (CoA) desaturase (SCD) enzymes are a family of δ-9 fatty acid desaturases that catalyse the de novo synthesis of monounsaturated fatty acids (MUFAs) from endogenous or dietary saturated fatty acids (SFAs). Here, we reported the loss of SCD1 leads to the down-regulation of B1 cells in the peritoneal cavity, providing a further insight on the role of SCD1 in B cell development. In this case, the comparison of B cell activation markers between the C57/BL6J and Scd1ab-2J/ab-2 B cells did not reveal any distinct differences. Also, further investigations into immunoglobulin production by B cells did not reveal significant differences between the C57/BL6J and Scd1f/fCd19Cre/+mice. However, Scd1f/fCd19Cre/+mice did show significant increased frequency of antibody-secreting cells in the spleen. The study in this work should allow us to gain a further insight on SCD1 role in B cell development and functions.