Development of chemical and enzymatic peptide ligation strategies

• Main Author: Cao, Yuan
• Other Authors: Liu Chuan Fa
• Format: Theses and Dissertations
• Language: English
• Published: 2015
• Subjects: DRNTU::Science::Biological sciences::Biochemistry
• Online Access: https://hdl.handle.net/10356/65380
• Institution: Nanyang Technological University

Proteins carry out virtually all the functions in almost every aspect of living organisms. To elucidate their roles in biological process, easy and convenient access to the proteins of interest is necessary. Chemical peptide synthesis and DNA-based recombinant technologies are the two major direct accesses to peptides/proteins. However, they have intrinsic limitations. Chemical synthesis has size limitation and DNA-based recombinant technology could not afford proteins with site-specific modifications. Peptide ligation strategies which enable the combined use of the above two would greatly expand the application scope. In my Ph.D research work, I mainly devoted to the development of peptide ligation strategies in both chemical and enzymatic manners.