A structural hypothesis for the transition between bent and extended conformations of the leukocyte β2 integrins

A structural hypothesis for the transition between bent and extended conformations of the leukocyte β2 integrins

In this thesis, three fragments (PHE1, PHE2 and PHE3) of integrin beta2 subunit have been expressed and crystallized for structural and functional studies. The following solved crystal structures of the beta2 Genu in both bent (PHE2) and extended (PHE3) conformations provide a structural basis, repr...

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Main Author: Shi, Minlong
Other Authors: Law Sai Kit, Alex
Format: Theses and Dissertations
Published: 2008
Subjects:
DRNTU::Science::Biological sciences
Online Access:https://hdl.handle.net/10356/6564
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-65642023-02-28T18:51:36Z A structural hypothesis for the transition between bent and extended conformations of the leukocyte β2 integrins Shi, Minlong Law Sai Kit, Alex School of Biological Sciences DRNTU::Science::Biological sciences In this thesis, three fragments (PHE1, PHE2 and PHE3) of integrin beta2 subunit have been expressed and crystallized for structural and functional studies. The following solved crystal structures of the beta2 Genu in both bent (PHE2) and extended (PHE3) conformations provide a structural basis, represented by conformational changes located in the beta2 Genu.In addition, we showed that LFA-1 can be locked into a bent conformation by a disulfide bond strategically introduced between the PSI and I-EGF2 domains, the “bent” LFA-1 has the capacity for the ligand binding. DOCTOR OF PHILOSOPHY (SBS) 2008-09-17T11:41:50Z 2008-09-17T11:41:50Z 2008 2008 Thesis Shi, M. (2008). A structural hypothesis for the transition between bent and extended conformations of the leukocyte β2 integrins. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/6564 10.32657/10356/6564 Nanyang Technological University application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
topic DRNTU::Science::Biological sciences
spellingShingle DRNTU::Science::Biological sciences
Shi, Minlong
A structural hypothesis for the transition between bent and extended conformations of the leukocyte β2 integrins
description In this thesis, three fragments (PHE1, PHE2 and PHE3) of integrin beta2 subunit have been expressed and crystallized for structural and functional studies. The following solved crystal structures of the beta2 Genu in both bent (PHE2) and extended (PHE3) conformations provide a structural basis, represented by conformational changes located in the beta2 Genu.In addition, we showed that LFA-1 can be locked into a bent conformation by a disulfide bond strategically introduced between the PSI and I-EGF2 domains, the “bent” LFA-1 has the capacity for the ligand binding.
author2 Law Sai Kit, Alex
author_facet Law Sai Kit, Alex
Shi, Minlong
format Theses and Dissertations
author Shi, Minlong
author_sort Shi, Minlong
title A structural hypothesis for the transition between bent and extended conformations of the leukocyte β2 integrins
title_short A structural hypothesis for the transition between bent and extended conformations of the leukocyte β2 integrins
title_full A structural hypothesis for the transition between bent and extended conformations of the leukocyte β2 integrins
title_fullStr A structural hypothesis for the transition between bent and extended conformations of the leukocyte β2 integrins
title_full_unstemmed A structural hypothesis for the transition between bent and extended conformations of the leukocyte β2 integrins
title_sort structural hypothesis for the transition between bent and extended conformations of the leukocyte β2 integrins
publishDate 2008
url https://hdl.handle.net/10356/6564
_version_ 1759858422911598592

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