Establishment and characterisation of CRTC1 knockout in rodent hippocampal neurons

• المؤلف الرئيسي: Koh, Tyler Bo Kai
• مؤلفون آخرون: Ch'ng Toh Hean
• التنسيق: Final Year Project
• اللغة: English
• منشور في: 2017
• الموضوعات: DRNTU::Science
• الوصول للمادة أونلاين: http://hdl.handle.net/10356/71555
• المؤسسة: Nanyang Technological University

CRTC1 is a transcriptional modulator that is crucial for learning and memory. At present, a conditional knockout mouse has never been studied either in neuronal cultures or live animals. In this report, we propose the use of a single lentiviral vector to deliver Cas9 and guide RNAs (gRNAs) into neur...