Optimizing directed evolution of S. pyogenes Cas9
CRISPR/Cas9, since its discovery, has revolutionized the genome editing landscape; with one protein and a programmable RNA sequence, genome targeting has become achievable even in the non-experts’ hands. Despite its easy adaptation of the application, off-target and low efficiency have been issues t...
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| Format: | Theses and Dissertations |
| Language: | English |
| Published: |
2017
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| Online Access: | http://hdl.handle.net/10356/72364 |
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| Institution: | Nanyang Technological University |
| Language: | English |