Optimizing directed evolution of S. pyogenes Cas9

• Main Author: Lee, Shi Mun
• Other Authors: Tan Meng How
• Format: Theses and Dissertations
• Language: English
• Published: 2017
• Subjects: DRNTU::Engineering::Bioengineering
• Online Access: http://hdl.handle.net/10356/72364
• Institution: Nanyang Technological University

CRISPR/Cas9, since its discovery, has revolutionized the genome editing landscape; with one protein and a programmable RNA sequence, genome targeting has become achievable even in the non-experts’ hands. Despite its easy adaptation of the application, off-target and low efficiency have been issues t...