Engineering of anti-cancer g-quadruplex nucleic acids and anti-bacterial protein fibers

Functional synthetic biopolymers are widely used in the therapeutic treatment and medical devices. This thesis consists of two parts focusing on nucleic acids and proteins respectively. In the first project, structural conformation and bioactivity of G-quadruplex-forming sequences, derived from a we...

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Bibliographic Details
Main Author: Truong, Thi Hong Anh
Other Authors: Phan Anh Tuan
Format: Theses and Dissertations
Language:English
Published: 2018
Subjects:
Online Access:http://hdl.handle.net/10356/73336
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Institution: Nanyang Technological University
Language: English
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Summary:Functional synthetic biopolymers are widely used in the therapeutic treatment and medical devices. This thesis consists of two parts focusing on nucleic acids and proteins respectively. In the first project, structural conformation and bioactivity of G-quadruplex-forming sequences, derived from a well-known aptamer AGRO100, was explored. Our findings on the novel G-quadruplex structures and their anti-proliferative activity against cancer cells might contribute in the development of oligonucleotides for therapeutic treatment. In the second project, a recombinant protein derived from elastin was synthesized and induced to self-assemble and form a fiber platform for silver nanoparticles formation. The silver-coated protein fibers exhibited the anti-bacterial properties on E. coli and S. aureus. This synthetic protein offers a novel biomaterial for an anti-bacterial coating in medical devices.