Structural plasticity of BCL-2 family proteins and its functional implication in apoptosis
Dysregulated apoptosis has been implicated in the development of several diseases including cancer and neurodegenerative diseases. Bcl-2 family proteins regulate mitochondrial apoptosis through homo- and hetero-dimerization between pro- and anti-apoptotic Bcl-2 proteins. The first part of this stu...
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| Format: | Theses and Dissertations |
| Language: | English |
| Published: |
2018
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| Online Access: | http://hdl.handle.net/10356/73522 |
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| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | Dysregulated apoptosis has been implicated in the development of several diseases including cancer and neurodegenerative diseases. Bcl-2 family proteins regulate mitochondrial apoptosis through homo- and hetero-dimerization between pro- and anti-apoptotic Bcl-2 proteins.
The first part of this study has been devoted to address the structural transition of Bcl-xL in forming domain-swapped dimer in the presence of n-octyl-β-D-Maltoside detergent. We postulated that this structural plasticity of Bcl-xL might play a regulatory role in the modulation of mitochondrial calcium homeostasis.
In the second part of this study, we focused on the molecular interaction between Bcl-xL and Voltage-Dependent Anion Channel (VDAC). While VDAC has been reported to interact with Bcl-xL to regulate mitochondrial calcium homeostasis, detailed molecular basis of the interaction between the two molecules remains elusive. Hence, we aim to provide structural insights into the interaction of Bcl-xL with VDAC N-terminal peptides using NMR spectroscopy. |
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