Alleviation of off-target effects from vector-encoded shRNAs via codelivered RNA decoys

• Main Authors: Mockenhaupt, Stefan, Grosse, Stefanie, Rupp, Daniel, Bartenschlager, Ralf, Grimm, Dirk
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2015
• Subjects: Adeno-associated viral vector; AAV; RNA interference; Short hairpin RNA; Off-targeting
• Online Access: https://hdl.handle.net/10356/81167; http://hdl.handle.net/10220/39147
• Institution: Nanyang Technological University

Exogenous RNAi triggers such as shRNAs ideally exert their activities exclusively via the antisense strand that binds and silences designated target mRNAs. However, in principle, the sense strand also possesses silencing capacity that may contribute to adverse RNAi side effects including off-target...