Alleviation of off-target effects from vector-encoded shRNAs via codelivered RNA decoys

• Main Authors: Mockenhaupt, Stefan, Grosse, Stefanie, Rupp, Daniel, Bartenschlager, Ralf, Grimm, Dirk
• 其他作者: School of Biological Sciences
• 格式: Article
• 語言: English
• 出版: 2015
• 主題: Adeno-associated viral vector; AAV; RNA interference; Short hairpin RNA; Off-targeting
• 在線閱讀: https://hdl.handle.net/10356/81167; http://hdl.handle.net/10220/39147
• 機構: Nanyang Technological University

Exogenous RNAi triggers such as shRNAs ideally exert their activities exclusively via the antisense strand that binds and silences designated target mRNAs. However, in principle, the sense strand also possesses silencing capacity that may contribute to adverse RNAi side effects including off-target...