A comparison and optimization of methods and factors affecting the transformation of Escherichia coli

• المؤلفون الرئيسيون: Verma, Chandra Shekhar, Lane, David P., Chan, Weng-Tat, Gan, Samuel Ken-En
• مؤلفون آخرون: School of Biological Sciences
• التنسيق: مقال
• اللغة: English
• منشور في: 2015
• الموضوعات: Competent bacteria; DMSO method; Hanahan’s method; CaCl2 method; Heat shock
• الوصول للمادة أونلاين: https://hdl.handle.net/10356/81298; http://hdl.handle.net/10220/39215
• المؤسسة: Nanyang Technological University

DNA manipulation routinely requires competent bacteria that can be made using one of numerous methods. To determine the best methods, we compared four commonly used chemical methods (DMSO, MgCl2–CaCl2, CaCl2 and Hanahan's methods) on frequently used Escherichia coli (E. coli) strains: DH5α, XL-...