A comparison and optimization of methods and factors affecting the transformation of Escherichia coli

• Main Authors: Verma, Chandra Shekhar, Lane, David P., Chan, Weng-Tat, Gan, Samuel Ken-En
• 其他作者: School of Biological Sciences
• 格式: Article
• 語言: English
• 出版: 2015
• 主題: Competent bacteria; DMSO method; Hanahan’s method; CaCl2 method; Heat shock
• 在線閱讀: https://hdl.handle.net/10356/81298; http://hdl.handle.net/10220/39215

DNA manipulation routinely requires competent bacteria that can be made using one of numerous methods. To determine the best methods, we compared four commonly used chemical methods (DMSO, MgCl2–CaCl2, CaCl2 and Hanahan's methods) on frequently used Escherichia coli (E. coli) strains: DH5α, XL-...