A comparison and optimization of methods and factors affecting the transformation of Escherichia coli

• Main Authors: Verma, Chandra Shekhar, Lane, David P., Chan, Weng-Tat, Gan, Samuel Ken-En
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2015
• Subjects: Competent bacteria; DMSO method; Hanahan’s method; CaCl2 method; Heat shock
• Online Access: https://hdl.handle.net/10356/81298; http://hdl.handle.net/10220/39215
• Institution: Nanyang Technological University

DNA manipulation routinely requires competent bacteria that can be made using one of numerous methods. To determine the best methods, we compared four commonly used chemical methods (DMSO, MgCl2–CaCl2, CaCl2 and Hanahan's methods) on frequently used Escherichia coli (E. coli) strains: DH5α, XL-...