Identifying and engineering promoters for high level and sustainable therapeutic recombinant protein production in cultured mammalian cells

Promoters are essential on plasmid vectors to initiate transcription of the transgenes when generating therapeutic recombinant proteins expressing mammalian cell lines. High and sustained levels of gene expression are desired during therapeutic protein production while gene expression is useful for...

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Main Authors: Ho, Steven C. L., Yang, Yuansheng
Other Authors: School of Chemical and Biomedical Engineering
Format: Article
Language:English
Published: 2016
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Online Access:https://hdl.handle.net/10356/81399
http://hdl.handle.net/10220/40783
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-813992023-12-29T06:45:47Z Identifying and engineering promoters for high level and sustainable therapeutic recombinant protein production in cultured mammalian cells Ho, Steven C. L. Yang, Yuansheng School of Chemical and Biomedical Engineering Promoter identification Recombinant protein production Mammalian cells Promoter engineering Promoters are essential on plasmid vectors to initiate transcription of the transgenes when generating therapeutic recombinant proteins expressing mammalian cell lines. High and sustained levels of gene expression are desired during therapeutic protein production while gene expression is useful for cell engineering. As many finely controlled promoters exhibit cell and product specificity, new promoters need to be identified, optimized and carefully evaluated before use. Suitable promoters can be identified using techniques ranging from simple molecular biology methods to modern high-throughput omics screenings. Promoter engineering is often required after identification to either obtain high and sustained expression or to provide a wider range of gene expression. This review discusses some of the available methods to identify and engineer promoters for therapeutic recombinant protein expression in mammalian cells. ASTAR (Agency for Sci., Tech. and Research, S’pore) Accepted version 2016-06-23T07:38:24Z 2019-12-06T14:30:06Z 2016-06-23T07:38:24Z 2019-12-06T14:30:06Z 2014 Journal Article Ho, S. C. L., & Yang, Y. (2014). Identifying and engineering promoters for high level and sustainable therapeutic recombinant protein production in cultured mammalian cells. Biotechnology Letters, 36(8), 1569-1579. 0141-5492 https://hdl.handle.net/10356/81399 http://hdl.handle.net/10220/40783 10.1007/s10529-014-1523-4 en Biotechnology Letters © 2014 Springer. This is the author created version of a work that has been peer reviewed and accepted for publication by Biotechnology Letters, Springer. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1007/s10529-014-1523-4]. 65 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Promoter identification
Recombinant protein production
Mammalian cells
Promoter engineering
spellingShingle Promoter identification
Recombinant protein production
Mammalian cells
Promoter engineering
Ho, Steven C. L.
Yang, Yuansheng
Identifying and engineering promoters for high level and sustainable therapeutic recombinant protein production in cultured mammalian cells
description Promoters are essential on plasmid vectors to initiate transcription of the transgenes when generating therapeutic recombinant proteins expressing mammalian cell lines. High and sustained levels of gene expression are desired during therapeutic protein production while gene expression is useful for cell engineering. As many finely controlled promoters exhibit cell and product specificity, new promoters need to be identified, optimized and carefully evaluated before use. Suitable promoters can be identified using techniques ranging from simple molecular biology methods to modern high-throughput omics screenings. Promoter engineering is often required after identification to either obtain high and sustained expression or to provide a wider range of gene expression. This review discusses some of the available methods to identify and engineer promoters for therapeutic recombinant protein expression in mammalian cells.
author2 School of Chemical and Biomedical Engineering
author_facet School of Chemical and Biomedical Engineering
Ho, Steven C. L.
Yang, Yuansheng
format Article
author Ho, Steven C. L.
Yang, Yuansheng
author_sort Ho, Steven C. L.
title Identifying and engineering promoters for high level and sustainable therapeutic recombinant protein production in cultured mammalian cells
title_short Identifying and engineering promoters for high level and sustainable therapeutic recombinant protein production in cultured mammalian cells
title_full Identifying and engineering promoters for high level and sustainable therapeutic recombinant protein production in cultured mammalian cells
title_fullStr Identifying and engineering promoters for high level and sustainable therapeutic recombinant protein production in cultured mammalian cells
title_full_unstemmed Identifying and engineering promoters for high level and sustainable therapeutic recombinant protein production in cultured mammalian cells
title_sort identifying and engineering promoters for high level and sustainable therapeutic recombinant protein production in cultured mammalian cells
publishDate 2016
url https://hdl.handle.net/10356/81399
http://hdl.handle.net/10220/40783
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