Identifying and engineering promoters for high level and sustainable therapeutic recombinant protein production in cultured mammalian cells

• Main Authors: Ho, Steven C. L., Yang, Yuansheng
• Other Authors: School of Chemical and Biomedical Engineering
• Format: Article
• Language: English
• Published: 2016
• Subjects: Promoter identification; Recombinant protein production; Mammalian cells; Promoter engineering
• Online Access: https://hdl.handle.net/10356/81399; http://hdl.handle.net/10220/40783
• Institution: Nanyang Technological University

Promoters are essential on plasmid vectors to initiate transcription of the transgenes when generating therapeutic recombinant proteins expressing mammalian cell lines. High and sustained levels of gene expression are desired during therapeutic protein production while gene expression is useful for...