Toward stable gene expression in CHO cells Preventing promoter silencing with core CpG island elements

• Main Authors: Mariati, Koh, Esther YC, Yeo, Jessna HM, Ho, Steven CL, Yang, Yuansheng
• Other Authors: School of Chemical and Biomedical Engineering
• Format: Article
• Language: English
• Published: 2016
• Subjects: CHO; core CpG island element; gene silencing; expression stability
• Online Access: https://hdl.handle.net/10356/81451; http://hdl.handle.net/10220/40784
• Institution: Nanyang Technological University

Maintaining high gene expression level during long-term culture is critical when producing therapeutic recombinant proteins using mammalian cells. Transcriptional silencing of promoters, most likely due to epigenetic events such as DNA methylation and histone modifications, is one of the major mecha...