Insertion of core CpG island element into human CMV promoter for enhancing recombinant protein expression stability in CHO cells

• Main Authors: Mariati, Yeo, Jessna H. M., Koh, Esther Y. C., Ho, Steven C. L., Yang, Yuansheng
• Other Authors: School of Chemical and Biomedical Engineering
• Format: Article
• Language: English
• Published: 2016
• Subjects: CHO; hCMV promoter; core CpG island element; gene silencing; expression stability; monoclonal antibody
• Online Access: https://hdl.handle.net/10356/81470; http://hdl.handle.net/10220/40790
• Institution: Nanyang Technological University

The human cytomegalovirus promoter (hCMV) is susceptible to gene silencing in CHO cells, most likely due to epigenetic events, such as DNA methylation and histone modifications. The core CpG island element (IE) from the hamster adenine phosphoribosyltransferase gene has been shown to prevent DNA met...