Evaluating the use of a CpG free promoter for long-term recombinant protein expression stability in Chinese hamster ovary cells

• Main Authors: Ho, S.C.L, Koh, E.Y.C, Soo, B.P.C, Chao, S.-H, Yang, Y
• Other Authors: MICROBIOLOGY AND IMMUNOLOGY
• Format: Article
• Published: 2020
• Subjects: Acetylation; Alkylation; Cells; Chromosomes; Clone cells; Cloning; Cytology; Genes; Genetic engineering; Mammals; Methylation; Proteins; Recombinant proteins; CHO cell; DNA Methylation; Gene silencing; Histone modification; Recombinant protein expression; Gene expression; dinucleotide; enhanced green fluorescent protein; histone; histone deacetylase inhibitor; messenger RNA; recombinant protein; animal cell; animal experiment; Article; cell clone; cell culture; CHO cell line; chromatin immunoprecipitation; chromosome; controlled study; CpG island; deacetylation; enhancer region; female; gene dosage; gene expression; gene interaction; gene repression; gene silencing; genetic analysis; genetic linkage; histone modification; intron; mouse; nonhuman; promoter region; protein expression; protein stability; animal; Cricetulus; drug stability; evaluation study; genetic enhancement; genetics; isolation and purification; metabolism; procedures; Animals; CHO Cells; CpG Islands; Drug Stability; Genetic Enhancement; Promoter Regions, Genetic; Recombinant Proteins
• Online Access: https://scholarbank.nus.edu.sg/handle/10635/181334
• Institution: National University of Singapore

10.1186/s12896-016-0300-y