Impact of Using Different Promoters and Matrix Attachment Regions on Recombinant Protein Expression Level and Stability in Stably Transfected CHO Cells

• Main Authors: Ho, Steven C. L., Mariati, Yeo, Jessna H. M., Fang, Shiyi Goh, Yang, Yuansheng
• Other Authors: School of Chemical and Biomedical Engineering
• Format: Article
• Language: English
• Published: 2016
• Subjects: Matrix attachment region (MAR); CMV promoter; SV40 promoter; CHO elongation factor 1α promoter; Stable recombinant protein expression; CHO cells
• Online Access: https://hdl.handle.net/10356/81465; http://hdl.handle.net/10220/40789
• Institution: Nanyang Technological University

High expression level and long-term expression stability are required for therapeutic protein production in mammalian cells. Three commonly used promoters from the simian virus 40 (SV40), the CHO elongation factor 1α gene (EF1α), and the human cytomegalovirus major immediate early gene (CMV) and two...