Butelase-mediated synthesis of protein thioesters and its application for tandem chemoenzymatic ligation
Using a recently discovered peptide ligase, butelase 1, we developed a novel method to access protein thioesters in good yield. We successfully combined it with native chemical ligation and sortase-mediated ligation in tandem for protein C-terminal labeling and dual-terminal labeling to exploit the...
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sg-ntu-dr.10356-818372023-02-28T16:58:26Z Butelase-mediated synthesis of protein thioesters and its application for tandem chemoenzymatic ligation Cao, Yuan Nguyen, Kien Truc Giang Tam, James P. Liu, Chuan-Fa School of Biological Sciences catalytic efficiency thioester Using a recently discovered peptide ligase, butelase 1, we developed a novel method to access protein thioesters in good yield. We successfully combined it with native chemical ligation and sortase-mediated ligation in tandem for protein C-terminal labeling and dual-terminal labeling to exploit the orthogonality of these three ligation methods. NRF (Natl Research Foundation, S’pore) Accepted version 2016-01-21T03:08:59Z 2019-12-06T14:41:14Z 2016-01-21T03:08:59Z 2019-12-06T14:41:14Z 2015 Journal Article Cao, Y., Nguyen, G. K. T., Tam, J. P., & Liu, C.-F. (2015). Butelase-mediated synthesis of protein thioesters and its application for tandem chemoenzymatic ligation. Chem. Commun., 51(97), 17289-17292. 1359-7345 https://hdl.handle.net/10356/81837 http://hdl.handle.net/10220/39726 10.1039/C5CC07227A en Chemical Communications © 2015 The Royal Society of Chemistry. This is the author created version of a work that has been peer reviewed and accepted for publication by Chemical Communications, The Royal Society of Chemistry. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1039/C5CC07227A]. 4 p. application/pdf |
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catalytic efficiency thioester |
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catalytic efficiency thioester Cao, Yuan Nguyen, Kien Truc Giang Tam, James P. Liu, Chuan-Fa Butelase-mediated synthesis of protein thioesters and its application for tandem chemoenzymatic ligation |
| description |
Using a recently discovered peptide ligase, butelase 1, we developed a novel method to access protein thioesters in good yield. We successfully combined it with native chemical ligation and sortase-mediated ligation in tandem for protein C-terminal labeling and dual-terminal labeling to exploit the orthogonality of these three ligation methods. |
| author2 |
School of Biological Sciences |
| author_facet |
School of Biological Sciences Cao, Yuan Nguyen, Kien Truc Giang Tam, James P. Liu, Chuan-Fa |
| format |
Article |
| author |
Cao, Yuan Nguyen, Kien Truc Giang Tam, James P. Liu, Chuan-Fa |
| author_sort |
Cao, Yuan |
| title |
Butelase-mediated synthesis of protein thioesters and its application for tandem chemoenzymatic ligation |
| title_short |
Butelase-mediated synthesis of protein thioesters and its application for tandem chemoenzymatic ligation |
| title_full |
Butelase-mediated synthesis of protein thioesters and its application for tandem chemoenzymatic ligation |
| title_fullStr |
Butelase-mediated synthesis of protein thioesters and its application for tandem chemoenzymatic ligation |
| title_full_unstemmed |
Butelase-mediated synthesis of protein thioesters and its application for tandem chemoenzymatic ligation |
| title_sort |
butelase-mediated synthesis of protein thioesters and its application for tandem chemoenzymatic ligation |
| publishDate |
2016 |
| url |
https://hdl.handle.net/10356/81837 http://hdl.handle.net/10220/39726 |
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1759854915148054528 |