Butelase-mediated synthesis of protein thioesters and its application for tandem chemoenzymatic ligation

• Main Authors: Cao, Yuan, Nguyen, Kien Truc Giang, Tam, James P., Liu, Chuan-Fa
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2016
• Subjects: catalytic efficiency; thioester
• Online Access: https://hdl.handle.net/10356/81837; http://hdl.handle.net/10220/39726
• Institution: Nanyang Technological University

Using a recently discovered peptide ligase, butelase 1, we developed a novel method to access protein thioesters in good yield. We successfully combined it with native chemical ligation and sortase-mediated ligation in tandem for protein C-terminal labeling and dual-terminal labeling to exploit the...