Native chemical ubiquitination using a genetically incorporated azidonorleucine

A robust chemical ubiquitination method was developed. The method employed a genetically incorporated azidonorleucine as an orthogonal lysine precursor for the installation of a Gly residue bearing an Nα-auxiliary which mediated the ligation between ubiquitin(1–75)-thioester and the target protein....

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Bibliographic Details
Main Authors: Yang, Renliang, Bi, Xiaobao, Li, Fupeng, Cao, Yuan, Liu, Chuan-Fa
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2016
Subjects:
Online Access:https://hdl.handle.net/10356/82021
http://hdl.handle.net/10220/39753
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Institution: Nanyang Technological University
Language: English