Native chemical ubiquitination using a genetically incorporated azidonorleucine

• Main Authors: Yang, Renliang, Bi, Xiaobao, Li, Fupeng, Cao, Yuan, Liu, Chuan-Fa
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2016
• Subjects: Biological Sciences
• Online Access: https://hdl.handle.net/10356/82021; http://hdl.handle.net/10220/39753
• Institution: Nanyang Technological University

A robust chemical ubiquitination method was developed. The method employed a genetically incorporated azidonorleucine as an orthogonal lysine precursor for the installation of a Gly residue bearing an Nα-auxiliary which mediated the ligation between ubiquitin(1–75)-thioester and the target protein....