Structural analysis and insights into the glycon specificity of the rice GH1 Os7BGlu26 β-D-mannosidase

• Main Authors: Robinson, Robert Charles, Tankrathok, Anupong, Iglesias-Fernández, Javier, Luang, Sukanya, Kimura, Atsuo, Rovira, Carme, Hrmova, Maria, Ketudat Cairns, James R.
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2016
• Subjects: Structural analysis; Glycoside hydrolases
• Online Access: https://hdl.handle.net/10356/82124; http://hdl.handle.net/10220/41104
• Institution: Nanyang Technological University

Rice Os7BGlu26 is a GH1 family glycoside hydrolase with a threefold higher kcat/Km value for 4-nitrophenyl β-D-mannoside (4NPMan) compared with 4-nitrophenyl β-D-glucoside (4NPGlc). To investigate its selectivity for β-D-mannoside and β-D-glucoside substrates, the structures of apo Os7BGlu26 at a re...