Structural analysis and insights into the glycon specificity of the rice GH1 Os7BGlu26 β-D-mannosidase

Rice Os7BGlu26 is a GH1 family glycoside hydrolase with a threefold higher kcat/Km value for 4-nitrophenyl β-D-mannoside (4NPMan) compared with 4-nitrophenyl β-D-glucoside (4NPGlc). To investigate its selectivity for β-D-mannoside and β-D-glucoside substrates, the structures of apo Os7BGlu26 at a re...

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Main Authors: Robinson, Robert Charles, Tankrathok, Anupong, Iglesias-Fernández, Javier, Luang, Sukanya, Kimura, Atsuo, Rovira, Carme, Hrmova, Maria, Ketudat Cairns, James R.
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2016
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Online Access:https://hdl.handle.net/10356/82124
http://hdl.handle.net/10220/41104
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Institution: Nanyang Technological University
Language: English

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